21 
DF214, a K-12 derivative f cxxitains Mu phage insertions in the £*iospho- 
glucose isomerase gene anc3 in an unidentified location. Dr. Goldstein 
said the probability of the Mu lysogen transducing out the rat DNA is very 
low and suggested the experiment be permitted. Dr. Gottesman agreed and 
suggested that the initial shotgun experiments screening the rat cDNA 
library be conducted at P2 physical ocxitainment. After the clone of 
interest has been selected, work may proceed at the PI level of containment. 
Dr. Goldstein moved acceptance of the proposal with Dr. Gottesman' s stipu- 
lation. Dr. Novick seconded the motion. 
By a vote of seventeen in favor, none opposed, and no abstentions, the 
RAC adc^ted the motion. 
XV. RBQUEgr FOR APPROVAL OF GUIDELINE CHANGES INVOLVING gTREPTOMYCES 
Dr. Levine introduced the proposal (tabs 1012, 1015/IV) submitted by 
Dr. Stanley Cohen of Stanford Uiiversity. Dr. Cc^en requested that 
Streptomyces ooelioolor and the related organisms with v^iich ^ coelicolor 
naturally exchanges genetic information (S^ lividans , S. ^rvulus , and 
S. griseus ) be approved as HVl hosts. Streptomyces plasmids SCP2, 
SLP1.2, pIJlOl, actinophage f*ii C31, and their derivatives would be used 
as vectors. 
Dr. Levine said the Streptomyces are ncxpathogenic; no known hazard has 
been associated with large-scale industrial use of the organisms. 
Q:. Levine moved approval of the proposal. Dr. Pedoroff seconded. 
Dr. Gottesman said the proposal was vague; the strains proposed for certi- 
fication are not as well defined as some of the HVL systems approved in 
the past. Little data concerning known exchange mechanisms with other 
organisms have been included in the pxroposal. Flirthermore, the actino- 
pAiages, v4iich are requested to be allowed as vectors, apparently have 
broad host ranges. 
As no further comment was made, Mr. Thornton called the vote. Ey a vote 
of ten in favor, none opposed, and eight abstentions, the RAC adopted the 
notion. 
Dr. Gottesman introduced the seccxid proposal (tabs 1012, 1015/V) from 
Dr. Oohen. Dr. Oohen requested that the following entry be added to 
^^pendix E: 
"Experiments involving the cloning of ENA among members of the 
genus Streptomyces are permitted under PI conditions. For these 
experiments, no registration document, as described in Part III, 
is required." 
Dr. Gottesman noted that entry 27 of ;^pendix E of the Guidelines currently 
permits clcxiing of ENA among members of the genus into ncxipethogenic Streg- 
tomyces under PI ocxitainment cxwxaitions. She said the request would extend 
[110] 
