22 
this by elijTiinating the requirement for a registration cJocurent. She felt 
that this eKTtion would be prenature; the working group evaluating the 
status of the Guidelines will be considering the need for registration 
docxments in genered. She moved to reject the request. Dr. Fedoroff 
seconded the motion. By a vote of fifteen in favor, none opposed, and 
three abstentions, RAC rejected Dr. Cohen's request. 
XVI. PROPOSED LARGE SCALE EXPE3U>1P/rS 
Dr. Pinon initiated the review of a proposal (tab 1001) from Dr. Barry Nall 
of the Uhiversity of Texas. Dr. Nall requested permission to perform 
large-scale fermentations of Sacchauxmyoes cerevisiae strains containing 
recoThinant DMA plasmids. The recombinant plasmid vectors consist of 
CNA from the ^ ooli plasmid pBR322, the yeast 2 micron circle, and yeast 
chromosanal DMA. Tfve Saccharomyoes cerevisiae cytochrome c gene will be 
ligated into these plasmid vectors. Dr. Pinon said the experiment is 
essentially self-cloning, and the sequences bo be cloned are well charac- 
terized. He noted that a registration docunent held not been submitted and 
suggested approval at Pl-LS containment be contingent upon submission of 
this document. He so moved. Dr. Williams seconded the motion. Dr. Qottesnan 
felt Pl-LS containment was not necessary and offered an amendment to substi- 
tute the words "good microbiological practice" for Pl-IS. Dr. McKinney 
disagreed, he felt Pl-LS actually represented "good microbiological prac- 
tice." Mr. Thornton called the vote on Dr. Gottesnan's proposed amendment. 
By a vote of four in favor, thirteen opposed, eind three abstentions, the 
RAC refused the proposed amendment. Mr. Thornton then czdled the vote on 
Dr. Pinon' s motion to approve the request at the Pl-LS level of containment. 
By a vote of nineteen in favor, none opposed, and no abstentions, the RAC 
adopted the motion. 
Mr. Thornton then c^Llled on Dr. Campbell to begin review of the proposal 
(tab 1018) from Dr. Hamilton O. Sh\ith of the Johns Hopkins university. 
Dr. Shiith requested permission to grow large quantities of the Hha II 
restriction and modification genes from Haemo^ilus haemolyticus , in 
E. ooli . Dr. Campbell said the DMA to be cloned in a pBR322 vector is a 
reasonably small, well-defined segment. He moved bo approve cloning of 
the plasmid in ^ ooli at the Pl-LS level of containment. By a vote of 
nineteen in favor, none opposed, and no abstentions, the RAC adopted the 
motion. 
XVII. REQUEST FX3R APPROVAL OF CERTAIN EXPERIMENTS DM3LVING ANABAEI^ AND NDgTOC 
Dr. Fedoroff introduced the proposal (tab 1013) of Dr. C. Peter Wblk of 
Michigan State Uhiversity. Dr. Wolk requested permission to (a) construct 
a recombinant molecule from an ^ coli plasmid and DMA from a strain of 
Anabaena , (b) propagate that DNA in an ooli K-12 strain, and (c) transfer 
the cloned DNA to a different Anabaena strain. DNA may also be derived 
from and/or transferred bo strains of the closely related genus. Nos toe . 
(1111 
