28 
PI ♦ HQ oontainroent for this type of experiment. He found PI + E3Q 
adequate for experiments 1 and 2, Dr. Holmes said ft:. Me3calanos had re- 
quested P2 HQ containment for experiment 3, but vould be ready to use 
P2 -f EJC2. Dr. Holmes felt experiment 3 could be performed under Pi EKl 
conditions as specified by the recently adopted toxin classification. 
Dr. Levine viewed the request as inportant work. He agreed with Dr. Holmes 
that PI EKl oontairrent conditions were appropriate for all three experi- 
ments. Dr. Holmes moved approval of experiments 1, 2, and 3 at the Pi + 
HQ level of oontairment. Dr. Levine seconded the motion. 
Dr. Novick suggested that P2 physical containment might be more appropriate 
to the experiments; he expressed oonoem that colonization factors, permit- 
ting ^ roli to attach to small bowel adhesion sites, might be picked up 
by ^ roli making cholera toxin. Cr. Levine said recent data have demon- 
strated that colonization factors edone will not create a pathogen. 
Dr. Nc^ick moved an amendment to require the experiments to be performed 
mder P2 containment conditions. He did not view P2 containment as an 
cxren4)elming burden and felt P2 would alleviate residu 2 Ll anxieties. 
Dr. Holmes said he would eKX»pt P2 requirements for experiment 3 but felt 
PI -f HQ was adequate for experiments 1 and 2. 
Dr. Levine replied that P2 oontainment would afford little or no exlditional 
safety since Vibrio cholerae is not spread by the aerosol route. As control 
of aerosol izat ion is the most significant distinction between PI and P2 
oontainment, he felt PI specifications are adequate to contain the experi- 
ments. He also pointed out that the general classification of toxins just 
appro/ed by the RAC permits the experiments at PI EKl. 
Dr. Holmes said he wished to rephrase his motion to make oontainment condi- 
tions contingent on the Director's decision concerning the general toxin 
classification (Appendix G); if Dr. Fredrickson accepts the general propo- 
sal, Dr. Nekedanos may proceed with experiments 1, 2, and 3 under PI contain- 
ment conditions. Should Dr. Fredrickson not accept the proposal, the RAC 
recommends that Dr. Mek^d^mo6 may perform experiments 1 and 2 under Pi 
conditions, but must use P2 oontainment for experiment 3. Dr. Levine, 
¥4x> had seconded Dr. Holmes' origin^d motion, did not accept the modified 
language; Kr. Thornton c^Llled the vote on the modified language. By a 
vote of three in favor, ten opposed, and three abstentions, the RAC refused 
the modified language. Mr. Thombon then c^dled the vote on Dr. Holmes' 
original langue^ge, i.e., appro/al of the proposed experiments at PI 
EKl oontaiment. By a vote of fifteen in favor, none opposed, and one 
abstention, the RAC adopted the motion. 
XX. QCNING AND E3(PRESSI0N OF CHA POPING FOR DIPHTHERIA TOXIN 
Dr. Holmes introduced the request (tab 1003, 1015/IX) of Cr. John Murphy 
of Harvard Lhiversity. Dr. Murphy proposed to clone in ^ ooli K-12 the 
3.9 kb Bam restriction fragment of Coryiephage Beta which carries the 
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