ATT7V3MNT 2 - PAGE 3 
Use tetanus teat in «4iich has no known effect on the gut itself r yet is 
exquisitely toxin in the circulation. Use piglets* 
(a) Dose lethality curves after direct injection of tetanus toxin into 
the patent gut, at the sites specified in 1 (b). Protect with 
tetanus antisenxn iv to chedc that death is caused by parenteral 
toxin. 
(b) Dose lethality curves in piglets with 
i) n^t^n/iral diarrhea, (v^ich daroages the gut histologically) 
ii) Secretory diarrhea caused by ^ ooli K80+ ent 
E. ooli K80+ ent gj« 
E. ooli K88+ ent- (control) 
iii) An invasive bacterial enteropathogen (e.£. , Yersinia, Capfjylobacter, 
Saliionella) 
iv) Outflow obstruction (which causes permeability to rise). Ligate guts 
at various levels 2 days previously, then inject tetanus toxin above 
the obstructions. 
(c) To learn something of the mechanism of trans- intestinal protein transport, 
repeat the dose-response curves of 2 (a) with 
i) Chlotoquin (prior to injection and every 12 h, p.o.) which 
blocks intestinal endocytosis 
ii) Verapamil, which blocks calcium channels and hence some transport. 
1131 ) 
