Federal Register / Vol. 46, No. 126 / Wednesday. July 1, 1981 / Notices 
34471 
be used for purified subgenomic DNA 
segments. (58) 
IlI-A-2-a-(l}-(a)-(2)-(b). P2 physical 
containment + an HV2 host-vector, or 
P3-»-HVl. shall be used for DNA 
recombinants produced with the whole 
viral genome or with subgenomic 
segments that have not been purified to 
the extent required in footnote 38. 
lU-A-2-a-{l)-{a}-{2}-{c). P2 physical 
containment -i- an HVl host-vector shall 
be used for DNA recombinants derived 
from purified cDNA copies of viral 
mRNA. (57) 
III-A-2-a-(lHa}-{5). Other 
Nontransforming Members of Presently 
Classified Viral Families. (36) 
IlI-A-2-a-{l)-{a)-{5)-{o). Pi physical 
containment + an HVl host-vector shall 
be used for (i) DNA recombinants 
produced with purified subgenomic 
DNA (58) segments or (fi) purified cDNA 
copies of viral mRNA. (57) 
lII-A-2-a-(l)-(a)-(5)^6). Pi physical 
containment + an HVl host-vector shall 
be used for DNA recombinants 
produced with the whole viral genome 
or with subgenomic segments that have 
not been purified to the extent required 
in footnote 38. 
ni-A-2-a-{l)-{b). Transforming 
Viruses. (37 A ) 
III-A-2-a-(l)-{b)-{7). Herpes Saimiri, 
Herpes Ate/es, and Epstein Barr Virus. 
(39) 
IlI-A-2-a-{l)-{b>-(lHo)- Fl physical 
containment -f- an HVl host-vector shall 
be used for DNA recombinants 
produced with purified nontransforming 
subgenomic DNA segments. (58) 
m-A-2-a-(lHl)H7HH P2 physical 
containment + an HVl host-vector shall 
be used for (i) DNA recombinants 
produced with purified subgenomic 
DNA segments containing an entire 
transforming gene [38] or (ii) purified 
cDNA copies of viral mRNA. (57) 
ni-A-2-a-(l)-(b)-(7)-(c). P3 physical 
containment -f an HVl host-vector, or 
P2-f HV2. shall be used for DNA 
recombinants produced with the whole 
viral genome or with subgenomic 
segments that have not been purified to 
the extent required in footnote 38. 
III-A-2-a-(lHbH2). Other 
Transforming Members of Presently 
Classified Viral Families. (36) 
UI-A-2-a-(l)-(b)-(2)-(o). Pi physical 
containment -f an HVl host-vector shall 
be used for DNA recombinants 
produced with purified nontransforming 
subgenomic DNA segments. (58) 
llI-A-2-a-(l)-(b)-(5)-(b). K physical 
containment -t- an HVl host-vector shall 
be used for (i) DNA recombinants 
produced with the whole viral genome, 
(ii) subgenomic DNA segments 
containing an entire transforming gene, 
(iii) purified cDNA copies of viral 
mRNA, (57) or (iv) subgeijomic segments 
that have not been purified to the extent 
required in footnote 58. 
lll-A-2-a-(2). DNA Transcripts of 
RNA Viruses. 
lII-A-2-a-(2)-(a). Retroviruses. 
UI-A-2-a-(2)-(a)-{7). Gibbon Ape, 
Woolly Monkey, Feline Leukemia and 
Feline Sarcoma Viruses. (39) 
llI-A-2-a-(2)-(a)-(7)-(o). Pi physical 
containment + an HVl host-vector shall 
be used for DNA recombinants 
produced with purified nontransforming 
subgenomic DNA segments. (58) 
IIl-A-2-a-(2)-(a)-(7)-(6). K physical 
containment + an HVl host-vector shall 
be used for DNA recombinants 
produced with purified subgenomic 
DNA segments (58) containing an entire 
transforming gene. 
IIl-A-2-a-{2)-{a)-(7)-{c). P2 physical 
containment -t- an HV2 host-vector, or 
P3 -t- HVl, shall be used for DNA 
recombinants produced with (i) the 
whole viral genome, (ii) purified cDNA 
copies of viral mRNA, (57) or (iii) 
subgenomic segments that have not 
been purified to the extent required in 
footnote 38. 
IH-A-2-a-(2)-(a)-(2). Other Members 
of the Family Retraviridiae. (36) 
llI-A-2-a-(2)-(a)-(5)-(a). Pi physical 
contairunent -t- an HVl host-vector shall 
be used for DNA recombinants 
produced with purihed nontransforming 
subgenomic DNA segments. (58) 
III-A-2-a-(2)-(a)-(5)-(f)). P2 physical 
containment -t- HVl host-vector shall be 
used for DNA recombinants produced 
with (i) subgenomic DNA segments 
containing an entire transforming gene, 
(ii) the whole viral genome, or (iii) 
purified cDNA copies of viral mRNA, 
(57) or (iv) subgenomic segments that 
have not been purified to the extent 
required in footnote 58. 
IlI-A-2-a-(2)-{b). Negative Strand 
RNA Viruses. PI physical containment 
+ an HVl host-vector shall be used for 
DNA recombinants produced with (i) 
cDNA copies of the whole genome, (ii) 
subgenomic cDNA segments, or (iii) 
purified cDNA copies of viral genome, 
(ii) subgenomic cDNA segments, or (iii) 
purified cDNA copies of viral mRNA. 
(57) 
IIl-A-2-a-(2}-(c). Plus-Strand RNA 
Viruses. 
lII-A-2-a-(2}-{c)-{7). Types 1 and 2 
Sabin Poliovirus Vaccine Strains and 
Strain 17D (Theiler) of Yellow Fever 
Virus. Pi physical containment -t- and 
HVl host-vector shall be used for DNA 
recombinants produced with (i) cDNA 
copies of the whole viral mRNA. (57) 
lll-A-2-a-(2)-(c)-^2). Other Plus- 
Strand RNA Viruses Belonging to 
Presently Classified Viral Families. (56) 
llI-A-2-a-(2)-^c)-(5)-(o). Pi physical 
containment + an HVl host-vector shall 
be used for DNA recombinants 
produced with purified subgenomic 
cDNA segments. (58) 
Table III . — Recommended Containment for Cloning of Viral DNA or cDNA in Certain HVl and 
HV2 Systems Specified in Appendix D 
I9xt for M det^s) 
Typo ol wral DNA sogment to bo donod 
Subgonorrac (59) 
GooorvK' 
No<ibViofO( niaig 
SogrDont 
oontBffw>g on 
onbro 
tronolorruing 
gono 
Nonsegmonted 
genome 
Segmented 
genome 
cONA from viral 
rriRNA (37) 
Nofitronofofmmg wuooo; 
AW. MVM. Mouoo 
P14-HV1 . 
PI 4 HVl - 
PI -f HVl. 
Adorx) {■trom FL). 
Plont Virxjooo 
P14-HV1 
PI + HVl.,.. 
Pl+HVt. 
HopotAuo B 
P1 +HV1 (3ffl 
P2 + HV2 0f 
.... P2 + HV1. 
PI +HV1 (J») 
P3 + HV1 
Pi +HV1 
PI +HV1. 
Tftnofomvng Vvuooo. 
Horpot, Ganrj H. 
PI HVl ( 
P2 + HV1 
P2 ^ HV2 or 
P2 + HV1. 
and EBV 
OttM. 
PI ♦ HVl (59) a. 
P2 + HV1 
P34HV1. 
P2 + HV1 
P2-FHV1. 
RNA 
RatrowuMS: 
GMxm Ap«. WooDy 
PI + HVl (39) 
P2 + HV1 ....„ 
P2 + HV2or 
P2 ^ HV2 Of 
Monkey FoLV or^t 
FoSV {39f. 
P1+HV1 (591 
Pi f HVl 
P2>HV1 
P34MV1 
P2 + HV1 
P3fHV1. 
P2 *^HV1 
Nogobvo-Skwvl 
P1+HV1 P1+HV1 
PI -► HVt. 
Pkjs-Slrand RNA. 
Typm 1 and 2 Salxn 
PoKo. 170 Yaaoar 
Few Vaccaia 
Strains 
Other ...._ 
P1-FHV1 
Pi 4HV1 
... . PI f HVl. 
PI . HVl (59) 
P2 + HV1 
[159] 
