34484 
Federal Regbter / Vol. 46, No. 126 / Wednesday, July 1, 1981 / Notices 
Coxsackie A and B viruses 
Cytomegolo viruses 
Echoviruses — all types 
Encephalomyocarditis virus (EMC) 
Flanders virus 
Hart Park virus 
Hepatitis-associated antigen material 
Herpes viruses — except Herpesvirus 
simiae (Monkey B virus) which is in 
Class 4 
Corona viruses 
Influenza viruses — all types except A/PR8/ 
34, which is in Class 1 
Langat virus 
Lymphogranuloma venereum agent 
Measles virus 
Mumps virus 
Parainfluenza virus — all types except 
Parainfluenza virus 3, SF4 strain, which 
is in Class 1 
Polioviruses — all types, wild and 
attenuated 
Poxviruses — all types except Alastrim, 
Smallpox, Monkey pox, and Whitepox, 
which depending on experiments, are in 
Class 3 or Class 4 
Rabies virus — all strains except Rabies 
street virus, which should be classified in 
Class 3 when inoculated into carnivores 
Reoviruses — all types 
Rubella virus 
Simian viruses — all types except 
Herpesvirus simiae (Monkey B virus) 
and Marburg virus, which are in Class 4 
Sindbis virus 
Tensaw virus 
Turlock virus 
Vaccinia virus 
Varicella virus 
Vole rickettsia 
Yellow fever virus, 17D vaccine strain 
C. Class 3 Agents 
1. Bacterial Agents: 
Actinobacillus mallei* 
Bartonella — all species 
Brucella — all species 
Francisella tularensis 
Mycobacterium avium, M. bovis, M. 
tuberculosis 
Pasteurella multocide type B ("buffalo” 
and other foreign virulent strains*] 
Pseudomonas pseudomallei* 
Yersenia pestis 
2. Fungal Agents: 
Coccidioides immitis 
Histoplasma capsulatum 
Histoplasma capsulatum var. duboisii 
3. Parasitic Agents; 
Schistosoma mansoni 
4. Viral, Rickettsial, and Chlamydial 
Agents: 
*** Alastrim, Smallpox, Monkey pox, and 
Whitepox, when used in vitro 
Arboviruses — all strains except those in 
Class 2 and 4 (Arboviruses indigenous to 
the United States are in Class 3, except 
those listed is Class 2. 
West Nile and Semliki Forest viruses may 
be classified up or down, depending on 
the conditions of use and geographical 
location of the laboratory.) 
Dengue virus, when used for transmission 
or animal inoculation experiments 
Lymphocytic choriomeningitis virus (LCM) 
Psittacosis-Ornithosis-Trachoma group of 
agents 
Rabies street virus, when used in 
inoculations of carnivores (See (Haas 2) 
Rickettsia — all species except Vole 
rickettsia when used for transmission or 
animal inoculations experiments 
Vesicular stomatitis virus * 
Yellow fever virus — wild, when used in 
vitro 
D. Class 4 Agents 
1. Bacterial Agents: None 
2. Fungal Agents: None 
3. Parasitic Agents: None 
4. Viral, Rickettsial, and Chlamydial 
Agents; 
*** Alastrim, Smallpox, Monkeypox, and 
Whitepox, when used for transmission or 
animal inoculation experiments 
Hemorrhagic fever agents, including 
Crimean hemorrhagic fever, (Congo), 
funin, and Machupo viruses, and oth» as 
yet undefined 
Herpesvirus simiae (Monkey B virus) 
Lassa virus 
Marburg virus 
Tick-borne encephalitis virus complex, 
including Russian spring-summer 
encephalitis, Kyasanur forest disease, 
Omsk hemorrhagic fever, and Central 
European encephalitis viruses 
Venezuelan equine encephalitis virus, 
epidemic strains, when used for 
transmission or animal inoculation 
experiments 
Yellow fever virus — wild, when used for 
transmission or animal inoculation 
experiments 
II. Classification of Oncogenic Viruses on the 
Basis of Potential Hazard [2) 
A. Low-Risk Oncogenic Viruses 
Reus Sarcoma 
SV-40 
CELO 
Ad7-SV40 
Polyoma 
Bovine papilloma 
Rat mammary tumor 
Avian Leukosis 
Murine Leukemia 
Murine Sarcoma 
Mouse mammary tumor 
Rat Leukemia 
Hamster Leukemia 
Bovine Leukemia 
Dog Sarcoma 
Mason-Pfizer Monkey Virus 
Marek's 
Guinea Pig Herpes 
Lucke (Frog) 
Adenovirus 
Shope Fibroma 
Shope Papilloma 
B. Moderate-Risk Oncogenic Viruses 
Ad2-SV40 
FeLV 
HV Saimiri 
EBV 
SSV-1 
GaLV 
HV ateles 
Yaba 
FeSV 
III. Animal Pathogens (d) 
A. Animal disease organisms which are 
forbidden entry into the United States by 
Law (CDC Class 5 agents) 
1. Foot and mouth disease virus 
B. Animal disease organiiuns and vectors 
which are forbidden entry into the 
United States by USDA Policy (CDC ^ 
Class 5 Agents) 
African horse sickness virus 
African swine fever virus 
Besnoitia besnoiti 
Boma disease vims 
Bovine infectious petechial fever 
Camel pox virus | 
Ephemeral fev«* virus 
Fowl plague virus 
Coat pox vims 
Hog cholera vims 
Looping ill virus 
Lumpy skin disease virus | 
Nairobi sheep disease virus ' 
Newcastle disease vims (Asiatic strains) 
Mycoplasma mycoides (contagious bovine 
pleuropneumonia] 
Mycoplasma agalactiae (contagions 
agalactia of sheep) 
Rickettsia ruminatium (heart water) 
Rift valley fever vims 
Rhinderpest virus 
Sheep pox virus 
Swine vesicular disease virus i 
Teschen disease virus 
Trypanosoma vivax (Nagana) ' 
Trypanosoma evansi 
Theileria parva (East Coast fever) 
Theileria annulate I 
Theileria lawrencei | 
Theileria bovis i 
Theileria hirci \ 
Vesicular exanthema vims j 
Wesselsbron disease vims I 
Zyonema 
Footnotes and References of Appenefix B | 
* A USDA permit required for import and 
interstate commerce of pathogens, may be 
obtained from the Animal and Plant Health ! 
Inspection Service, USDA, Federal Building, 
Hyattsville, MD. 20782. 1 1 
**Since the publication of the classification I 
in 1974 [1], the Actinomycetes have been ' i 
reclassified as bacterial rather than fungal 
agents. 
* * * All activities, including storage of 
variola and whitepox are restricted to the 
single national facility (WoHd Health 
Organization (WHO) Collaborating Center 1 1 
for Smallpox Research, Center for Disease ' 
Control, in Atlanta). 
(1) Classification of Etiologic Agents on the j 
Basis of Hazard. (4th Edition, July 1974). U.S. i' 
Department of Health. Education and k ^ 
Welfare, Public Health Service, Center for -i 
Disease Control, Office of Biosafety, Atlanta, | 
Georgia 30333. i j 
[2] National Cancer Institute Safety 
Standards for Research Involving Oncogenic u , 
Viruses (Ottober 1974), U.S. Department of I ; 
Health, Education, and Welfare Pubhcaticui } ^ 
No. (NIH) 78-790. 
(J) U.S. Department of Agriculture. Animal ^ g 
and Plant Health Inspection Service. f g 
[172] 
