21 
Dr. Bems pointed out that industry currently ooonplies with the NIH Guide- 
lines voluntarily; there is no roandatory requirement for industrial firms 
to institute IBCs. Dr. McKinney concurred but added that industry is 
complying out of self-interest as well as public interest. In view of RAC's 
experience with these three host-vector systems, he said the committee should 
recommend Dr. Johnson's proposal. 
Mr. Ihomton called the vote. ^ a vote of eleven in favor, two opposed, 
and cxie abstention, the RAC adopted the proposal. 
X. PROPOSAL TO INCLUDE STREPTOCOCCUS PYOGENES ON SUBLIST F OF APPENDIX A 
Mr. 'Ihomton asked Dr. Maas to begin discussion of the proposal (tabs 1028, 
1035/8) from Dr. Joseph Ferretti of the ttiiversity of Oklahoma Health Sciences 
Center to include Streptococcus pyogenes on sublist F of ^^pendix A. In 
support of his request. Dr. Ferretti submitted evidence demonstrating genetic 
exchange between Streptococcus pyogenes and Streptococcus sanguis . Strep- 
tococcus sanguis is currently included in sublist F . 
Dr. Maas said the data demonstrate genetic exchange between ^ pyogenes and 
S. sanguis . These exchanges occur primarily through transformation and 
conjugation with plasmids. Dr. Maas felt the request was reasonable and 
moved approval. Dr. Fedoroff seconded the motion. 
Dr. Goldstein asked if Streptococcus pyogenes is iirplicated in rheumatic fever. 
Dr. Maas replied that it was. However, it was agreed that ^ pyogenes merited 
inclusion on Sublist F of /^pendix A on the basis of exchange data. 
Mr. Thornton called for the vote on the motion. ^ a vote of fourteen in 
favor, none opposed, and no abstentions, the RAC adopted the proposal. 
XI. REQUEST TO PERMIT ONE-WAY TRANSFER OF STREPTOCOCCUS LACTIS ENA INTO 
S. SANGUIS AND TO PERMIT TRANSFER OF A RECOMBINANT PLASMID FROM S. FAECALIS 
TO S. LACTIS 
Dr. Fedoroff introduced the request (tabs 1029, 1035/3) of Dr. Larry McKay 
of the University of Minnesota for permission to transfer Streptococcus 
lactis KIA into Streptococcus sanguis strain Challis. Dr. McKay also 
requested that these strains be included in ^^pendix A on the basis that 
they exchange genetic information by known physiological processes. In 
addition, he requested permission to transfer a recombinant plasmid fron 
faecal is to lactis . 
Dr. Fedoroff noted that Dr. McKay wishes to reduce S. lactis plasmids in 
size with endonucleases in order to obtain the smallest functional plasmid. 
He would then purify the plasmid, transfer it to Streptococcus sanguis by 
transformation, transfer it by conjugation from sanguis to S. faecalis , 
and return it to S. lactis from £. faecalis by conjugation. 
[212] 
