22 
Dr. Fedoroff asked if organians have been inclixJed in Appendix A of the Guide- 
lines on the basis of data denonstrating i^idirectional treinsfonnation. 
Dr. Gartland said organians had been included in Appendix A on that basis. 
Dr. Fedoroff, on the basis of the data submitted, moved approval of the 
requests as written. Dr. McGarrity seconded the motion. 
Dr. Ahned requested a clarification of the motion; he asked if S. lactis 
and S. s^u^is would be placed on an existing sublist of AppendTx A or if 
a new sublist would be created. Dr. Gartland asked Dr. Fe^roff whether 
the submitted evidence justifies including S. lactis in Sublist F of 
Appendix A. Dr. Fedoroff said the evidence supports one way transformation 
of S. sanguis by S. lactis ENA, but not the reverse. Dr. Talbot suggested, 
therefore, that Scblist E of Appendix A might appropriately be amended to 
permit transformation of S. sanguis by S. lytis ENA. A new entry could 
also be added to Appendix E to permit transfer of a recombinant plasmid from 
S. faecal is bo S. lactis by conjugation. By a vote of fourteen in favor, 
none opposed, and no eibs tent ions, the RAC approved these actions. 
XII. REQUEST TO CLONE SACCHARCMYCES CEREVISIAE ENA IN SALMONELLA TYPHIMURIEM 
Dr. Pinon introduced the request (tabs 1031, 1035/5, 1039) of Drs. Christopher 
Marvel and Edward Penhoet of the Uhiversity of California, Berkeley, to clone 
Saccharomyces cerevisiae ENA in Salmonella typhimuriun , using a nonnobilizable 
plaamid (YEpl3). 
Dr. Pinon noted that S. typhimuriun is a CDC Class 2 etiological agent, but 
the investigators wilT enploy attenuated strains. Furthermore, ENA from S. 
cerevisiae , a nonpathogen, will be introduced. Dr. Pinon reoommended that 
the investigators be permitted to proceed under PI containment conditions. 
Dr. Maas concurred and moved acceptance of the request. Dr. Pinon seconded 
the motion. 
Dr. Abned asked why Drs. Marvel and Penhoet suggest they might be willing to 
erploy P3 containment conditions. Dr. Landy said he did not feel willingness 
to employ high containment indicated the investigators have concerns on the 
safety of the experiments, but rather an eagerness to begin the research and 
a willingness to work under RAC imposed conditions. 
Mr. Thomton called the question. By a vote of fourteen in favor, none 
opposed, and no abstentions, RAC adopted the motion to permit Drs. Marvel and 
Penhoet to proceed under Pi containment conditions. 
( 213 ) 
