Attachnent III - Page 4 
Revision of the July 1^ 1981 Guidelines Pressed by Working Group on 
Revision of the Guidelines ^ July 9^ 1981 
(1) Section I-A of the Guidelines would be amended to read as follows: 
I-A, Purpose . The purpose of these Guidelines is to specify standard 
practices for constructing and handling (i) reccxnbinant molecules 
and (ii) organisms and viruses containing recombinant ENA molecules. 
(2) Section I-D of the Guidelines, Prohibitions , would be eliminated. 
(3) Part III of the Guidelines would be replaced with the following language: 
Part III discusses experiments covered by the Guidelines. The reader 
should first consult Part I, vAiere exenpt experiments are listed. 
Where recommended physical containment levels applicable to non-recembinant 
DNA experiments exist for either the host or the vector (such as those 
specified by the CDC Guidelines, or the USDA Quarantine Regulations), 
recombinant ENA experiments should be carried out at containment levels 
at least as high as those recommended for non-recombinant DNA experiments. 
If there is clear evidence that the donor ENA will significantly change 
the pathogenicity of the host, the containment level ^propriate to the 
anticipated change will be applied. Otherwise, all experiments may be 
carried out under conditions of PI or Pl-LS physical containment. 
(4) Those membership specifications of the IBC to be found in Section IV-D 
of Section IV of the Guidelines would be eliminated. 
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