Federal Register / Vol. 46, No. 233 / Friday, December 4, 1981 / Notices 
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8. Pseudomonas aeruginosa, Pseudomonas 
putida and Pseudomonas fluorescens 
9. Serratia marcescens 
Subiisi B 
1. Bacillus sub tills 
2. Bacillus licheniformis 
3. Bacillus pumilus 
4. Bacillus globigii 
5. Bacillus niger 
6. Bacillus nato 
7. Bacillus amyloiiquefaciens 
8. Bacillus aterrimus 
Sublist C 
1. Streptomyces aureofaciens 
2. Streptomyces rimosus 
3. Streptomyces coelicolor 
Subiist D 
1. Streptomyces griseus 
2. Streptomyces cyaneus 
3. Streptomyces venezuelae 
Sublist E 
One way transfer of Streptococcus mutans or 
Streptococcus lactis DNA into 
Streptococcus sanguis. 
Sublist F 
1. Streptococcus sanguis 
2. Streptococcus pneumoniae 
3. Streptococcus faecalis 
4. Streptococcus pyogenes 
Appendix B. — ClassiHcation of 
Microorganisms on the Basis of Hazard 
I. Classification of Etiologic Agents on 
the Basis of Hazard (1) 
A. Class 1 Agents 
All bacterial, parasitic, fungal, viral, 
rickettsial, and chlamydial agents not 
included m higher classes. 
B. Class 2 Agents 
1. Bacterial Agents 
Actinobacillus — all species except A. mallei, 
which IS in Class 3 
Arizona hmshawii — all serotypes 
Bacillus anthracis 
Bordetella — all species 
Borrelia recurrentis, B. vincenti 
Clostridium botulinum. 
Cl. chauvoei. Cl. haemolyticum. 
Cl. histolyticum. Cl. novyi. 
Cl. septicum. Cl. tetani 
Corynebacterium diptheriae, 
C. egui, C. haemolyticum 
C. pseudotuberculosis 
C. pyogenes, C. renale 
Diplocococcus (Streptococcus) pneumoniae 
Erysipelothrix insidiosa 
Escherichia coli — all enteropathogenic 
serotypes 
Haemophilus ducreyi, H. influenzae 
Herellae vaginicola 
Klebsiella — all species and all serotypes 
Leptospira interrogans — all serotypes 
Listeria — all species 
Mima poloymorpha 
Moraxella — all species 
Mycobacteria — all species except those 
listed in Class 3 
Mycoplasma — all species except 
Myaoplasma mycoides and Mycoplasma 
agalactiae, which are in Class 5 
Neisseria gonorrhaeae, N. meningitidis 
Pasteurella — all species except those listed in 
Class 3 
Salmonella — all species and all serotypes 
Shigella — all species and all serotypes 
Sphaerophorus necrophorus 
Staphylococcus aureaus 
Streptobacillus moniliformis 
Streptococcus pyogenes 
Treponema carateum, T pallidum, and T 
pertenue 
Vibrio fetus, V comma, including biotype El 
Tor, and V parahemolyticus 
2. Fungal Agents 
**Actinomycetes (including Nocardia species 
and Actinomyces species and Arachnia 
propionicaj 
Blastomyces dermatitidis 
Cryptococcus neoformans 
Paracoccidioides brasiliensis 
3. Parasitic Agents 
Endomoeba histolytica 
Leishmania sp. 
Naegleria gruberi 
Toxoplasma gondii 
Toxocara canis 
Trichinella spiralis 
Trypanosoma cruzi 
4. Viral, Rickettsial, and Chlamydial Agents 
Adenoviruses — human — all types 
Cache Valley virus 
Coxsackie A and B viruses 
Cytomegloviruses 
Echoviruses — all types 
Encephalomyocarditis virus (EMC) 
Flanders virus 
Hart Park virus 
Hepatitis-associated antigen material 
Herpes viruses — except Herpesvirus simiae 
(Monkey B virus) which is in Class 4 
Corona viruses 
Influenza viruses — all types except A/PR8/ 
34, which is in Class 1 
Langat virus 
Lymhogranuloma venereum agent 
Measles virus 
Mumps virus 
Parainfluenza virus — all types except 
Parainfluenza virus 3, SF4 strain, which is 
in Class 1 
Polioviruses — all types, wild and attenuated 
Poxviruses — all types except Alastrim, 
Smallpox, Monkey pox, and 'Whitepox, 
which depending on experiments, are in 
Class 3 or Class 4 
Rabies virus — all strains except Rabies street 
virus, which should be classified in Class 3 
when inoculated into carnivores 
Reoviruses — all types 
Respiratory syncytial virus 
Rhinoviruses — all types 
Rubella virus 
Simian viruses — all types except Herpesvirus 
simiae (Monkey B virus) and Marburg 
virus, which are in Class 4 
Sindbis virus 
Tensaw virus 
Turlock virus 
Vaccinia virus 
Varicella virus 
Vole rickettsia 
Yellow fever virus, 17D vaccine strain 
C. Class 3 Agents 
t Bacterial Agents 
Actinobacillus mallei * 
Bartonella — all species 
Brucella — all species 
Francisella tularensis 
Mycobacterium avium, M. bovis, M. 
tuberculosis 
Pasteurella multocide type B ("buffalo" and 
other foreign virulent strains*) 
Pseudomonas pseudomallei * 
Yersenia pestis 
2. Fungal Agents 
Coccidioides immitis 
Histoplasma capsulatum 
Histoplasma capsulatum var. duboisii 
3. Parasitic Agents 
Schistosoma mansoni 
4. Viral, Rickettsial, and Chlamydial Agents 
* * * Alastrim, Smallpox, Monkey pox, and 
Whitepox, when used in vitro 
Arboviruses — all strains except those in 
Class 2 and 4 (Arboviruses indigenous to 
the United States are in Class 3, except 
those listed is Class 2. 
West Nile and Semliki Forest viruses may be 
classified up or down, depending on the 
conditions of use and geographical location 
of the laboratory.) 
Dengue virus, when used for transmission or 
animal inoculation experiments 
Lumphocytic choriomeningitis virus (LCM) 
Psittacosis-Ornithosis- Trachoma group of 
agents 
Rabies street virus, when used m 
inoculations of carnivores (See Class 2) 
Rickettsi—a\\ species except Vole rickettsia 
when used for transmission or animal 
inoculation experiments 
Vesicular stomatitis virus * 
Yellow fever virus — wild, when used in vitro 
D. Class 4 Agents | 
1: Bacterial Agen ts fl j 
None ? 
2. Fungal Agents Ij 
None . 
3. Parasitic Agents (j 
None 
4. Viral, Rickettsial, and Chlamydial Agents '• 
* * 'Alastrim, Smallpox, Monkey pox, and 
Whitepox, when used for transmission or ( 
animal inoculation experiments | 
Hemorrhagic fever agents, including Crimean i 
hemorrhagic fever, (Congo), funin, and 
Machupo viruses, and others as yet t 
undefined ' 
Herpesvirus simiae (Monkey B virus) 4 
Lassa virus 
Marburg virus I 
Tick-borne encephalitis virus complex, 
including Russian spring-summer i j 
encephalitis, Kyasanur forest diesase, I ( 
Omsk hemorrhagic fever, end Central i j 
European encephalitis viruses ! Ij 
Venezuelan equine encephalitis virus, f (j 
epidemic strains, when used for 
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