59384 Federal Register / Vol. 4G, No. 233 / Friday. December 4. 1981 / Notices 
OUTLINE Oe CURHO/T NIH GUItCLINtS TOK K£XX>lBIt<IANr DNA RESEARCH AND PROPOSED CHANGES 
Current Guideline s; 
Procedures 
Subooniiiittee 
Proposal 
Balti/nore-Campbell 
Proposal 
I. Prohibitions (I-O) 
CDC aass 4(5 
organisns 
Genes for potent 
toxins 
Release to 
environment 
Genes for drug 
resistance 
10 liters mless 
well characterized 
^md harmless 
Prohibitions take 
precedence over 
all otlter parts of 
guidelines; 
exceptions possible 
by application to 
RAC and Federa l 
Register publicat ion 
This {xrohiLition does 
not take precedence over 
exfxrptions 1-4 
Eliminate prohibitions 
as special class; 
handled by mC as 
with other experiments 
Retains prohibitions 
II. 
Exenptlons (1-E) 
Naked CNA 
Rearrangements J 
f No special oversight 
reguired for tl«se 
Self-cloning 1 
experiments; new 
additions to list 
Exchangers / 
by application to 
RAC and publication 
J 
Others: Tissue 1 
in Federal le^ister 
it: 
culture, E. ooli, | 
D. subtills and 1 
S. oerevisiae HVl / 
systems ' 
• 
III. 
HVl Systems (TII-A) 
Qirrently covers 
J Containmr.rit varies 
only some N. crassa 
f with source of donor 
and Streptomyevs 
\ rWA (P1-P3). 
strains 
1 Niw rystems rojuire 
V apprt>val by KM' ami 
1 Feieral (k^ister 
’ publicat icn 
IV. All other 
P3» lexer by request 
PI for 
PI for 
prokaryote, lower 
to ORIA. 
nonpathogens; 
ncxijiathorjens 
eukaryote 
Return to hist of 
igthORTis as per 
etc. 
corbinations 
origin, PI 
CTX:, etc. 
V. Eukaryote 
recipients 
y 
r 
r non-viral vectors 
P1-P2 in wtolc organ. 
Pi for non- 
PI for non- 
(exempt in tifisue culture) 
pathogens, etc. 
pathogens, etc. 
defective viral 
Pl-helper, P2-P3 ♦ helper 
► vectors 
1 nun-defect ive 
case-hy-case; in most 
PI for non- 
Pi for non- 
viral wetors 
cases) 
paOxjgeas, etc. 
patliogens, etc. 
Prooslural Changes: IBC imn(>ersliip six^cifiel 
■UJNQ coot «iio-o«-c 
no specifications 
for membership 
voluntary system 
