59392 
Federal Register / Vol. 46. No. 233 / Friday. December 4. 1981 / Notices 
(5) Maintaining prereview of all non- 
exempt experiments by the IBC. It is 
proposed that the requirements for the 
constitution of the IBC be dropped while 
the essential element of consultation 
with a group other than the principal 
investigator is retained. 
Refereocei 
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(r.*?) Federal Register (July 7 1976). 
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27902-27943. 
[20) Federal Register (July 28. 1978). 
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[21] Federal Register ()uly 28 1978). 
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Guidelines for Research Involving 
Recombinant DNA Molecules. 60108-60131. 
[23) Federal Register (July 1, 1981). 
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(47) Workshop on Recombinant DNA Risk 
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Recombinant DNA Technical Bulletin, Vol, 4, 
No. 3. 98-120. 
Apiyendix A of Annex B: Experiments 
with E. coli 
One of the apprehensions of scientists 
early in the recombinant DNA era was 
that certain recombinant organisms 
(particularly Escherichia coli) might 
inadvertantly escape from the 
laboratory, particularly by way of 
inadvertant colonization of laboratory 
personnel. Furthermore it was feared 
that temporary intestinal colonization of 
a laboratory worker might result in 
exchange of genetic information from 
the laboratory E. coli strain to normal 
intestinal coliform flora. It was also 
feared by some that since some E. coli 
strains are pathogenic for man, perhaps 
E. coli K-12 should be regarded as a 
pathogen or “potential" pathogen. 
Most of the early work with 
recombinant DNA involved E. coli K-12. 
Since the genetics of this strain are so 
well-studied, much (if not most] 
recombinant DNA research still involves 
derivatives of this strain. Thus it is 
[281] 
