59408 
Federal Register / Vol. 46, No. 233 / Friday, December 4. 1981 / Notices 
that have not been puriHed to the extent 
required in footnote 38. 
ni-A-2-a-{2). DSA Transcripts of 
RNA Viruses. 
IIl-A-2-a-{2)-{a)- Retroviruses. 
in-A-2-a-(2HaHl)- Gibbon Ape, 
Woolly Monkey, Feline Leukemia and 
Feline Sarcoma Viruses. [39j 
III-A-2-a-(2)-{aHlHo). Pi physical 
containment + an HVl host-vector shall 
be used for DNA recombinants 
produced with purified nontransforming 
subgenomic DNA segments.(38] 
III-A-2-a-{2HaHlH^)- P2 physical 
containment + an HVl host-vector shall 
be used for DNA recombinants 
produced with purified subgenomic 
DNA 8egments[38] containing an entire 
transforming gene. 
III-A-2-a-{2HaHfH<^)- P2 physical 
containment -f an HV2 host-vector, or 
P3 HVl, shall be used for DNA 
recombinants produced with (i) the 
whole viral genome, (li) puriHed cDNA 
copies of viral mRNA.(37j or (iii) 
subgenomic segments that have not 
been puri6ed to the extent required in 
footnote 38. 
III-A-2-a-{2HaHf)- Other Members 
of the Family Retroviridiae.[36] 
III-A-2-a-^2)-(aH'^Ho). Pi physical 
containment -t- an HVl host-vector shall 
be used for DNA recombinants 
produced with purified nontransforming 
subgenomic DNA segments.[38] 
Ul-A-2-a-{2)-(aH^?H^)- P2 physical 
containment -f an HVl host-vector shall 
be used for DNA recombinants 
produced with (i) subgenomic DNA 
segments containing an entire 
transforming gene, (ii) the whole viral 
genome, or (iii) purifled cONA copies of 
viral mRNA.[37] or (iv) subgenomic 
segments that have not been purified to 
the extent required in footnote 38. 
Ul-A-2-a-(2)-{b). Negative Strand 
RNA Viruses. physical containment 
-f- an HVl host-vector shall be used for 
DNA recombinants produced with (i) 
cONA copies of the whole genome, (ii) 
subgenomic cDNA segments, or (iii) 
puribed cDNA copies of viral 
mRNA.(37] 
in-A-2-a-(2)-(c). Plus-Strand RNA 
Viruses. 
III-A-2-a-(2)-(c)-(7). Types 1 and 2 
Sabin Poliovirus Vaccine Strains and 
Strain 17D (Theiler) of Yellow Fever 
Virus. Pi physical containment and 
HVl host-vector shall be used for DNA 
recombinants produced with (i) cDNA 
copies of the whole viral genome, (ii) 
subgenomic cDNA segments, or (iii) 
purified cDNA copies of viral 
mRNA.(37) 
Ul_A-2-a-(2Hc)-(2). Other Plus- 
Strand RNA Viruses Belonging to 
Presently Classified Viral Families.[36j 
IlI-A-2-a-(2}-{c)-(2)-(o). Pi physical 
containment 4 an HVl host-vector shall 
be used for DNA recombinants 
produced with purified subgenomic 
cDNA segments.(38] 
Table III.— Recommended Containment fon Cloning of Viral DMA or cONA in Certain mvi and HV2 Systems Specified in Appendix 0 
Wu« CtM 
Typd ol vM ONA (dgnranl to b* cun«d 
SueganondcCSS) 
Oman*:* 
cONA from virol 
mRNA(37] 
- -■ - ocvitMrwv 
PiOrWWlWTTWlQ MVTWw .■ 
Sagmamad ganama 
ONA 
NonVirnAymng onjMC 
AAV. MVM. Mouw Adano iSVan 
R1*MV1 
Pi -f MVI . 
PI +MV1. 
FU 
VInjM* - J 
^ -.MW1 
Pi 4. HVl 
PI +HV1. 
HMMi 0 - . .. 
P®*f MV? ^ P?4.HV1 ^ 
P2 + MV1 
P1+HV1 
P2 + HV1. 
P2 + HV1 
P2 + MV2 or P3 + HV1. 
P2 + HV1. 
P1+HV1. 
PI 4- MVI. 
P24MV1. 
PI 4>HV1. 
rmrne 
PI 4. HVl 
Tranatonnmg WuMC 
SanwL H Ai«m »id 
EavTss) 
OWr ... 
1 
R1 ...MVI rial 
1 
R2 ♦ MVI ■■ H 
P2*f HV2 or P3-f.MV1 
n 
P34.HV1 
RNA 
WWotln— : 
OMon Ap*. MooSy Mami«y 
F«LV ird F«ev(3Sl 
rXtMr 
| 
1 
P24-KV2 or P34*HV1 
^4-HV1(30] ^ 
P3-4.HV1 
P2 4^HV1 
Olr^ WNA ^ 
^4. HVl 
PI 4. HVl 
Pl^HVI 
RNA 
1 md 2 Sdbx Rolo. *70 
Vatar Fmt vaaxn* SVMna. 
Orm . _ 
*MV1 
Pi 4. MVI 
Pl4.HVir311 
P2 4.HV1 
OnutN 9»r«nd«d RNA . . . 
P14.HV1 __i 
Pl^HVI 
M WiAdi , Ri .^HVi 4 
mncaduw Viral ONA i Sm m .. ^Smioi _h 
R1.1.MV1 JP1.1.MV1 
s***" r - - - 
- - - 1 
P1+HV1. 
L 
*Sm « w pSen gN«n m wiarMi m Ntfat AcpanSi Q. 
UI-A-2-a-(2}-(c)-(.2)-{Z)). P2 physical 
containment 4 an HVl host-vector shall 
be used for DNA recombinants 
produced with (i) cDNA copies of the 
whole genome, or (ii) purified cDNA 
copies of viral mRNA.(37] 
in-A-2-a-(2)-(d). Double-Stranded 
Segmented RNA Viruses. Pi physical 
containment 4- an HVl host-vector shall 
be used for DNA recombinants 
produced with (i) mixtures of 
subgenomic cDNA segments, (ii) a 
specific subgenomic cDNA segment, or 
(iii) purified cONA copies of viral 
mRNA.(37) 
IU-A-2-a-(2)-(e). RNA Plant Viruses 
and Plant Viroida.{A&\ Pi physical 
containment 4 - an fWl host-vector shall 
be used for DNA recombinants 
produced %vith (i) cDNA copies of the 
whole viral genome, (ii) subgenomic 
cDNA segments, or (iii) purified cDNA 
copies of viral mRNA.[37l 
IlI-A-2-a-(3). Intracellular Viral 
DNA. Physical and biological 
containment specified for shotgun 
experiments with eukaryotic cellular 
DNA [see Section m-A-(l)-(a)] shall be 
used for DNA recombinants produced 
with integrated viral DNA or viral 
genomes present in infected cells. 
ni-A-2-b. Eukaryotic Organelle 
DNAs. P2 physical containment 4- an 
HVl host-vector, or Pi 4 HV 2 . for 
mitochondrial or chloroplast DNA from 
eukaryotes when the organelle DNA has 
been obtained from isolated organelles. 
Otherwise, the conditions given for 
shotgun experiments apply. 
in-A-2-c. Prokaryotic Plasmid and 
Phage DNAs. The containment levels 
required for shotgun experiments with 
DNA from prokaryotes apply to their 
[297] 
