Federal Register / Vol. 46, No. 233 / Friday, December 4, 1981 / Notices 
59421 
Parainfluenza virus — all types except 
Parainfluenza virus 3, SF4 strain, which is 
in Class 1 
Polioviruses — all types, wild and attenuated 
Poxviruses — all types except Alastrim, 
Smallpox, Monkey pox, and Whitepox, 
which depending on experiments, are in 
Class 3 or Class 4 
Rabies virus — all strains except Rabies street 
virus, which should be classified in Class 3 
when inoculated into carnivores 
Reoviruses — all types 
Respiratory syncytial virus 
Rhinoviruses — all types 
Rubella virus 
Simian viruses — all types except Herpesvirus 
sirniae (Monkey B virus) and Marburg 
virus, which are in Class 4 
Sindbis virus 
Tensaw virus 
Turlock virus 
Vaccinia virus 
Varicella virus 
Vole rickettsia 
Yellow fever virus, 17D vaccine strain 
C. Class 3 Agents 
1. Bacterial Agents 
Actinobacillus mallei* 
Bartonella — all species 
Brucella — all species 
Francisella tularensis 
Mycobacterium avium, M. bovis, M. 
tuberculosis 
Pasteurella muitocide type B ("buffalo” and 
other foreign virulent strains*) 
Pseudomonas pseudomallei* 
yersenio postis 
2. Fungal Agents 
Coccidioides immitis 
Histoplasma capsulatum 
Histoplasma capsulatum var. duboisii 
3. Parasitic Agents 
Schistosoma mansoni 
4. Viral, Rickettsial, and Chlamydial Agents 
*** Alastrim, Smallpox, Monkey pox, and 
Whitepox, when used in vitro 
Arboviruses — all strains. except those in 
Classes 2 and 4 (Arboviruses indigenous to 
the United States are in Class 3, except 
those listed in Class 2. West Nile and 
Semliki Forest viruses may be classi6ed up 
or down, depending on the conditions of 
use and geographical location of the 
laboratory.) 
Dengue virus, when used for transmission or 
animal inoculation experiments 
Lymphocytic choriomeningitis virus (LCM) 
Psittacosis-Ornithosis-Trachoma group of 
agents 
Rabies street virus, when used in 
inoculations of carnivores (See Class 2) 
Rickettsia — all species except Vole rickettsia 
when used for transmission or animal 
inoculation experiments 
Vesicular stomatitis virus * 
Yeliow fever virus — wild, when used in vitro 
D. Class 4 Agents 
I. Bacterial Agents 
None 
3. Fungal Agents 
None 
3. Parasitic Agents 
None 
Viral, Rickettsial, and Chlamydial Agents 
* * * Alastrim. Smallpox, Monkey pox. and 
Whitepox, when used for transmission or 
animal inoculation experiments 
Hemorrhagic fever agents, including Crimean 
hemorrhagic fever, (Congo), Junin, and 
Machupo viruses, and others as yet 
undefined 
Herpesvirus sirniae (Monkey B virus) 
Lassa virus 
Marburg virus 
Tick-borne encephalitis vims complex, 
including Russian spring-summer 
encephalitis, Kyasanur forest disease, 
Omsk hemorrhagic fever, and Central 
European encephalitis vimses 
Venezuelan equine encephalitis virus, 
epidemic strains, when used for 
transmission or animal inoculation 
experiments 
Yellow fever vims — wild, when used for 
transmission or animal inoculation 
experiments 
II. Classification of Oncogenic Viruses 
on the Basis of Potential Hazard (2) 
A. Low-Risk Oncogenic Viruses 
Rous Sarcoma 
SV-10 
CELO 
Ad7-SV40 
Polyoma 
Bovine papilloma 
Rat mammary tumor 
Avian Leukosis 
Murine Leukemia 
Murine Sarcoma 
Mouse mammary tumor 
Rat Leukemia 
Hamster Leukemia 
Bovine Leukemia 
Dog Sarcoma 
Mason-Pfizer Monkey Virus 
Marek’s 
Guinea Pig Herpes 
Lucke (Frog) 
Adenovirus 
Shope Fibroma 
Shope Papilloma 
B. Moderate-Risk Oncogenic Viruses 
Ad2-SV40 
FeLV 
HV Saimiri 
EBV 
SSV-1 
GaLV 
HV ateles 
Yaba 
FeSV 
III. Animal Pathogens (3) 
A. Animal disease organisms which are 
forbidden entry into the United States by Law 
(CDC Class 5 agents) 
1. Foot and mouth disease virus 
B. Animal disease organisms and vectors 
which are forbidfden entry into the United 
States by USDA Policy (CDC Class 5 Agents) 
African horse sickness virus 
African swine fever virus 
Besnoitia besnoiti 
Borna disease virus 
Bovine infectious petechial fever 
Camel pox virus 
Ephemeral fever virus 
Fowl plague virus 
Goat pox virus 
Hog cholera virus 
Louping ill virus 
Lumpy skin disease virus 
Nairobi sheep disease virus 
Newcastle disease virus (Asiatic strains) 
Mycoplasma mycoides (contagious bovine 
pleuropneumonia) 
Mycoplasma agalactiae (contagious agalactia 
of sheep) 
Rickettsia ruminatium (heart water) 
Rift valley fever virus 
Rhinderpest virus 
Sheep pox virus 
Swine vesicular disease virus 
Teschen disease virus 
Trypanosoma vivax (Nagana) 
Trypanosoma evansi 
Theileria parva (East Goast fever) 
Theileria annulata 
Theileria lawrencei 
Theileria bovis 
Theileria hirci 
Vesicular exanthema virus 
Wesselsbron disease virus 
Zyonema 
Footnotes and References of Appendix B 
*A USDA permit, required for import and 
interstate commerce of pathogens, may be 
obtained from the Animal and Plant Health 
Inspection Service, USDA, Federal Building, 
Hyattsville, MD. 20782. 
** Since the publication of the classification 
in 1974 [1], the Actinomycetes have been 
reclassiOed as bacterial rather than fungal 
agents. 
***A11 activities, including storage of 
variola and whitepox are restricted to the 
single national facility (World Health 
Organization (WHO) Collaborating Center 
for Smallpox Research, Center for Disease 
Control, in Atlanta). 
(7) Classification ofEtiologic Agents on the 
Basis of Hazard. (4th Edition, July 1974). U.S. 
Department of Health, Education and 
Welfare, Public Health Service, Center for 
Disease Control, Office of Biosafety, Atlanta, 
Georgia 30333. 
[2] National Cancer Institute Safety 
Standards for Research Involving Oncogenic 
Vimses (October 1974). U.S. Department of 
Health, Education, and Welfare Publication 
No. (NIH) 75-790 
(.?) U.S. Department of Agriculture, Animal 
and Plant Health Inspection Service. 
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