20 
Dr. Maas asked how the FMIV strains cloned by Molecular Genetics, Inc., 
differed fron the strains cloned by Genentech, Inc. Ur. Pilacinski said the 
FMW strains cloned by Molecular Genetics, Inc., are indigenous to Argentina 
and latin America. 
Et. Ahmed asked why Molecular Genetics, Inc., had requested PI containment 
conditions for the work in Minnesota, cr. Bems said that PI conditions 
had been previously appro/ed for the stbgenonic FMIV clone work conducted 
by Genentech, Inc., in California. Cr. Goldstein asked v^t scale experi- 
ments Molecular Genetics anticipated. Dr. Collett said the work would be 
laboratory scale. 
Dr. Bems moved approval of the request. Dr. McKinney seconded the motion. 
Dr. Tadbot clarified the language of the motion: the motion would provide 
for review of IBDA infectivity data by the FMIV working Gkroup of the RAC 
before NIH permission would be granted. Ihis procedure had been followed 
in approving the Genentech, Inc., request. Dr. Goldstein inquired about 
the canpoeition of this working group. Dr. Qartland said Drs. Baltimore, 
Bems, and Tblin currently compose the group, and additionad members will 
be named. Dr. Goldstein said he wished that the decision of the working 
group be sent to the RAC. Dr. Bems agreed. 
Mr. Thornton cadled the vote. By a vote of sixteen in favor, none opposed, 
and four abstentions, the motion was approved. 
VI. PROPOSED PCUSICN OF YERSINIA EyTEROCOLITICA CN SDBUgT A OF APPENDIX A 
Dr. FWoroff said that tabs 1052 and 1056C/6 present a request fron 
Dr. Guy Cornells of the Lhiversite Catholique de Louvain, Brussels, 
Belgium. Dr. Cornel is requested that Yersinia enterooolitica be exempted 
from the Guidelines u^r Section I-C-4 and added to Siblist A, Appendix A. 
Dr. Pedoroff said ^ enterocoliti^ ecch 2 mges genetic information with 
E. ooli with a frequency of tr 2 msfer roughly three orders of magnitude 
IcMer than seen in exchange between E. ooli and ^ ooli . Uhder certain 
conditions, that frequency can be enJnanced. FiJtants vinicb ha\« higher 
exchange frequencies can also be selected. 
Dr. Fedoroff asked Dr. Levine to coiment on Yersinia enterooolitica . 
Dr. Levine said that some strains of Yersinia enterooolitica cause 
disease in man. The disease producing serotypes are invasive, with sane 
producing a heat stable enterotoxin whose mech^mi3m of action is identical 
to that of heat stable ^ ooli enterotoxin. In school age children, Y. 
enterooolitica is a major cause of mesenteric adenitis vfiich leads to a 
pseudo-appendicitis type syndrcxre. In older individuals one sees hyper- 
sensitivity reactions, including erythema nodosum; in individuals of the 
HIAV27 allotype, chronic arthritis may develcp following Yersinia infection. 
(3491 
