21 
Er. Levine said the data denonstrate genetic exchange with ^ coli in the 
test tube, and this exchange probably occurs in nature. On that basis, he 
supported the proposal. Er. Fedor off noved ^proval of the proposal. 
Dr. Levine seconded the motion. By a vote of eighteen in fevor, none 
opposed, and one abstention, the RAC approved the rooticxi. 
VII. ITOPOSED PSEUDOMJJAS PUTIEA HOST-VECTOR SYSTEM 
Dr. Maas introduced the proposal (tabs 1053, 1056C/5) of Er. Michael 
Bagdasarian of the Max-Planck Institut fur Molekulare Qenetik, Berlin, 
West Germany. Er. Bagdasarian requested HVl certification of a host-vector 
system based on Pseudoronas putida strain KT2440 and plasmid cloning vectors 
pKT262, pKT263 and pKT264. 
Dr. Maas questioned v^iether it is appropriate fiar RAC to consider a proposal 
whioh originated with an investigator outside the U.S. Mr. Thornton 
replied that if the ^stem could be widely applied in research, RAC might 
appropriately evaluate it for certificaticxi. Drs. Maas, Fedoroff, and 
McKinney said a P^ putida host-vector ^stem could be widely used . 
Er. Maas said the informaticxi provided supports the investigator's request 
for HVl c:ertification, and so moved. Dr. Bems seconded the motion, adding 
that a P^ ^tida HVl system would be very useful as genes which are not 
expressed in ^ coli host-vector systems may be expressed in P^ putida 
systems. Mr. Thornton called the moticxi. By a vote of eighteen in favor, 
none cpposed, and one abstention, the RAC apprcved the motion. 
VIII. PROPOSAI5 INVOLVING EK2 HOST-VECTOR SYSTEMS 
A. Proposed EK2 Host-Vector Systans 
Dr. Friednan introduced the prcposal (tdDS 1054, 1056C/3) of Er. Roy 
Curtiss of the Lhiversity of Alabama, Birmingham, Alabaira. Er. Curtiss 
requested EK2 certification of six different ^ coli K-12 strains in 
conjunction with various virulent and temperate bactericphage lanbda, 
plasmid, and cosmid vectors. Dr. Curtiss also requested that all 
previously approved EK2 vectors be approved as vector components of the 
proposed EK2 host-vector ^sterns. 
Dr. Friedman said the proposed EK2 host-vector systems are: 
(1) ^ coli K-12 x 2447, and its suppressor- free sib x2281, for 
use with virulent bacteriophage lambda vectors including 
specifically, but not limited to, Charon 4A. 
[350] 
