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Federal Register / VoL 47, No. 60 f Monday, March 29, 1981 f Notices 
**44. DNA from the chloroplaets of pliants 
may be cloned in the cyanobacterium 
Anacystis niduhna strain R-2 under Pi 
containment conditions using the plasmid 
vector pUCl04.” 
F. Certification of Pseudomonas 
Putida HVl Host-Vector System. The 
following paragraph is added under the 
heading “HVl” in Appendix F of the 
Guidelines: 
“Pseudomonas putida strain KT2440 with 
plasmid vectors pKT262, pKT2e3, and pKT264 
is certified as an HVl host-vector system.” 
G. Certification of EK2 Host-Vector 
Systems. The following paragraphs are 
added to Appendix F of the Guidelines 
under the heading ' EK2 Bacteriophage 
Systems 
“E. coli K-12 strains chi-2447 and chi-2281 
are certified for use v/ith lambda vectors that 
are certified for use with strain DP50 or 
DPSOsupP provided that the su* strain not be 
used as a propagation host. 
“E. coli K-12 strains chi-1984, chi-2705, chi- 
2001, and chi-2363 are certifled for use with 
lambda vectors that are certifled for use with 
strain DP50 or DPSOsupP provided that the 
su** not strains be used as propagation hosts.” 
Note. — OMB's “Mandatory Information 
Requirements for Pederal Assistance Program 
Announcements” (45 PR 39592) requires a 
statement concerning the official government 
programs contained in the Catalog of Federal 
Domestic Assistance. Normally NIH lists in 
its announcements the number and title of 
aflected individual programs for the guidance 
of the public. Because the guidance in this 
notice covers not only virtually every NIH 
program but also essentially every federal 
research program in which DNA recombinant 
molecule techniques could be used, it had 
been determined to be not cost effective or in 
the public interest to attempt to list these 
programs. Such a list would likely require 
several additional pages. In addition, NIH 
could not be certain that every federal 
program would be included as many federal 
agencies, as well as private organizations, 
both national and international, have elected 
to follow the NIH Guidelines. In lieu of the 
individual program listing, NIH invites 
readers to direct questions to the information 
address above about whether individual 
programs listed in the Catalog of Federal 
Domestic Assistance are affected. 
NIH programs are not covered by 0MB 
Circular A-95 because they fit the description 
of “program not considered appropriate” in 
Section 8-(b)-(4) and (5) of that Circular. 
Dated: March 18, 1982. 
Bernard Talbot, 
Acting Director, National Institute of Allergy 
and Infectious Diseases, National Institutes of 
Health. 
|FR Doc. 82-8108 Filed 3-26-82; 8:45 am] 
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