Federal Register / Vol. 47, No. 102 / Wednesday, May 26, 1982 / Notices 
23125 
Influenza viruses — all types except A/PR8/ 
34, which is in Class 1 
Langat virus 
Lymphogranuloma venereum agent 
Measles virus 
Mumps virus 
Parainfluenza virus — all types except 
Parainfluenza virus 3, SF4 strain, which 
is in Class 1 
Polioviruses — all types, wild and 
attenuated 
Poxviruses — all types except Alastrim, 
Smallpox, and Whitepox, which are 
Class 5 and Monkpy pox, which 
depending on experiments, is in Class 3 
or Class 4 
Rabies virus — all strains except Rabies 
street virus, which should be classified in 
Class 3 
Reoviruses — all types 
Respiratory syncytial virus 
Rhinoviruses — all types 
Rubella virus 
Simian viruses— b\\ types except 
Herpesvirus simiae (Monkey B virus) 
and Marburg virus, which are in Class 4 
Sindbis virus 
Tensaw virus 
Turlock virus 
Vaccinia virus 
Varicella virus 
Vesicular stomatitis virus [3] 
Vole rickettsia 
Yellow fever virus, 17D vaccine strain 
Appendix B-I-C. Class 3 Agents 
Appendix B-I-C-1. Bacterial Agents 
Actinobacillus mallei [3] 
Bartonella — all species 
Brucella — all species 
Francisella tularensis 
Mycobacterium avium, M. bovis, M. 
tuberculosis 
Pasteurella multocide type B ("buffalo” 
and other foreign virulent strains [3] 
Pseudomonas pseudomallei [3] 
Yersinia pestis 
Appendix ^I-C-2, Fungal Agents 
Coccidioides immitis 
Histoplasma capsulatum 
Histoplasma capsulatum var, duboisii 
Appendix B-I-C-3. Parasitic Agents 
Schistosoma mansoni 
Appendix B-I-C-4, Viral, Rickettsial, and 
Chlamydial Agents 
Monkey pox, when use in vitro [4] 
Arboviruses — all strains except those in 
Class 2 and 4 (Arboviruses indigenous to 
the United States are in Class 3, except 
those listed in Class 2. West Nile and 
Semliki Forest viruses may be classified 
up or down, depending on the conditions 
of use and geographical location of the 
laboratory.) 
Dengue virus, when used for transmission 
or animal inoculation experiments 
Lymphocytic choriomeningitis virus (LCM) 
Psittacosis-Omithosis-Trachoma group of 
agents 
Rabies street virus 
Rickettsia — all species except Vole 
rickettsia when used for transmission or 
animal inoculation experiments 
Yellow fever virus — wild, when used in 
vitro 
Appendix B-I-D. Class 4 Agents 
Appendix B-l-D-1. Bacterial Agents. None 
Appendix B-I-D-2. Fungal Agents. None 
Appendix B-I-D-3. Parasitic Agents. None 
Appendix B-I-D-4. Viral, Rickettsial, and 
Chlamydial Agents 
Monkey pox, when used for transmission 
or animal inoculation experiments [4] 
Hemorrhagic fever agents, including 
Crimean hemorrhagic fever, (Congo), 
Junin, and Machupo viruses, and others 
as yet undeflned 
Herpesvirus simiae (Monkey B virus) 
Lassa virus 
Marburg virus ' 
Tick-borne encephalitis virus complex, 
including Russian spring-summer 
encephalitis, Kyasanur forest disease, 
Omsk hemorrhagic fever, and Central 
European encephalitis viruses 
Venezuelan equine encephalitis virus, 
epidemic strains, when used for 
transmission or animal inoculation 
experiments 
Yellow fever virus — wild, when used for 
transmission or animal inoculation 
experiments 
Appendix B-II. Classification of Oncogenic 
Viruses on the Basis of Potential Hazard 
[5] 
Appendix B-II-A. Low-Risk Oncogenic 
Viruses. These viruses should be treated 
as Class 2 agents 
Rous Sarcoma 
SV-40 
CELO 
Ad7-SV40 
Polyoma 
Bovine papilloma 
Rat mammary tmbr 
Avian Leukosis 
Murine Leukemia 
Murine Sarcoma 
Mouse mammary tumor 
Rat Leukemia 
Hamster Leukemia 
Bovine Leukemia 
Dog Sarcoma 
Mason-Pfizer Monkey Virus 
Marek's 
Guinea Pig Herpes 
Lucke (Frog) 
Adenovirus 
Shope Fibroma 
Shope Papilloma 
Appendix ^D-B. Moderate-Risk Oncogenic 
Viruses. These viruses should be treated 
as Class 3 agents 
Ad2-SV40 
FeLV 
HV Saimiri 
EBV 
SSV-1 
GaLV 
HV ateles 
Yaba 
FeSV 
Appendix B-III. Class 5 Agents 
Appendix B-III-A. Animal Disease 
Organisms Which Are Forbidden Entry 
into the United States by Law 
Foot and mouth disease virus 
Appendix B-IU-B. Animal Disease 
Organisms and Vectors Which Are 
Forbidden Entry into the United States 
by USDA Policy 
African horse sickness virus 
African swine fever virus 
Besnoitia besnoiti 
Borna disease virus 
Bovine infectious petechial fever 
Camel pox virus 
Ephemeral fever virus 
Fowl plague virus 
Goat pow virus 
Hog cholera virus 
Louping ill virus 
Lumpy skin disease virus 
Nairobi sheep disease virus 
Newcastle disease virus (Asiatic strains) 
Mycoplasma mycoides (contagious bovine 
pleuropneumonia) 
Mycoplasma agalactiae (contagious 
agalactia of sheep) 
Rickettsia ruminatium (heart water) 
Rift valley fever virus 
Rhinderpest virus 
Sheep pox virus 
Swine vesicular disease virus 
Teschen disease virus 
Trypanosoma vivax (Nagana) 
Trypanosoma evansi 
Theileria parva (East Coast fever) 
Theileria annulata 
Theileria lawrencei 
Theileria bovis 
Theileria hirci 
Vesicular exanthema virus 
Wesselsbron disease virus 
Zyonema 
Appendix B-III-C. Organisms Which May 
Not Be Studied in the United States 
Except At Specified Facilities 
Small pox [4] 
Alastrim [4] 
White pox [4] 
Appendix B-IV. Footnotes and References of 
Appendix B 
1. The original reference for tfiis 
classification was the publication 
Classification ofEtiologic Agents on the 
Basis of Hazard, 4th edition, July 1974, U.S. 
Departmet of Health, Education, and Welfare, 
Public Health Service, Center for Disease 
Control, Office of Biosafety, Atlanta, Georgia 
30333. For the purposes of these Guidelines, 
this list has been revised by the NIH. 
2. Since the publication of the classiflcation 
in 1974 [1], the Actinomycetes have been 
reclassified as bacterial rather than fungal 
agents. 
3. A USDA permit, required for import and 
interstate transport of pathogens, may be 
obtained from the Animal and Plant Health 
Inspection Service, USDA, Federal Building, 
Hyattsville, MD 20782. 
4. All activities, including storage of variola 
and whitepox are restricted to the single 
national facility [World Health Organization 
(WHO) Collaborating Center for Smallpox 
Research, Center for Disease Control, in 
Atlanta). 
5. National Cancer Institute Safety 
Standards for Research Ivolving Oncogenic 
Viruses (October 1974). U.S. Department of 
Health, Education, and Welfare Publication 
No. (NIH) 75-790. 
6. U.S. Department of Agriculture, Animal 
and Plant Health Inspection Service. 
Appendix C. — Exemptions Under ni-D-5 
Section III-D-5 states that exempt from 
these Guidelines are “Other classes of 
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