TUFTS UNIVERSITY 
School of Medicine 
Department of Molecular Biology and 
Microbiology 
March 24, 1981 
Dr. Elizabeth Milewski 
ORDA-NIH 
Bldg. 31, Rm. 4A52 
Bethesda, MD 20205 
Dear Dr. Milewski: 
I habe been informed by Dr. Donald H. Dean that 
he has requested specific changes in the NIH Guide- 
lines for Recombinant DMA Research with respect to 
the use of Bacillus subtilis as a cloning system. I 
strongly endorse those specific changes as a means 
of bringing the B. subtilis system into greater use 
in recombinant DNA work. 
Sincerely yours 
ALS : ms 
136 Harrison Avenue 
Boston, Massachusetts oztii 
617 956-6750 
[ 572 ] 
