MESNA 24 mg/kg IV over 30 minutes at 0,3,6,9,12,15 
hours post-cyclophosphamide, (see appendix for detailed 
drug information) 
DAY 2 Begin G-CSF at a dose of 12 ug/kg/day 
subcutaneously, (see appendix for 
... detailed drug information) 
When total white blood cell count recovers to > 
1000/ul: begin daily leukophereses , continue until at 
least 3 X 10 8 nucleated cells/kg are collected. G-CSF 
will be discontinued after the final leukopheresis , or 
when the total circulating white blood cell count 
reaches 20,000/ul. 
5.3 5-Fluorouracil Priming of Marrow 
At least 14 days after discontinuation of G-CSF and 
completion of leukopheresis, a bone marrow aspirate and 
biopsy will be performed. -If the peripheral blood 
absolute neutrophil, count is < 1500/ul or the platelet 
count is < 100 , 000/ul at this point, then the repeat 
bone marrow aspirate and biopsy will be delayed until 
the peripheral counts reach those levels. If 
cellularity is greater than 30 %, the patient will be 
given 5-f luorouracil 15 mg/kg IV qd X 3 (see appendix 
for detailed drug information). If the cellularity is 
less than 30%, the 5FU therapy will be delayed until 
cellularity is greater than 30% Bone marrow aspirate 
and biopsy will be performed on day 5 and on day 15 
following the initial dose of 5-f luorouracil to assess 
cellularity, progenitor content and retroviral 
inf ectivity . 
5.4 Bone Marrow Harvest 
See section 5.2. Bone marrow harvest will occur on day 
10 after the first dose of 5-f luorouracil , and no later 
than day -5 prior to transplantation (see below). Bone 
marrow harvest will be delayed if the absolute 
neutrophil count is below 1000/ul or the platelet count 
.is below 100,000 until the peripheral blood counts 
recover to this level. 
5.5' High-Dose Chemoradiotherapy and Transplant 
Throughout patients -will receive standard antiemetic 
regimens as required, and intravenous hydration to 
maintain good urine output and normalize electrolytes. 
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Recombinant DNA Research, Volume 16 
