media is added in equal amounts to the cell suspension 
on ice. Then the freezing bags containing the mixture 
are placed in a Cryo-Med Control Rate Freezer and 
cooled at a 1° C/minute down to -30° C. At that time, 
the freezing rate is increased to 10° C. /minute until 
a temperature of -90° C is reached. Then the freezing 
bags are removed and placed into a liquid nitrogen 
storage freezer. 
8 . 0 PRESTUDY EVALUATION 
8.1 Clinical evaluation 
1. Complete history and physical examination, with 
height, weight and body surface area calculations 
2. Dental evaluation. 
3. Chest X-ray, and full bone survey if not performed 
within three months of admission. Other X-rays as 
clinically indicated. 
4. Electrocardiogram, gated blood pool scan (MUGA) 
5. Pulmonary function tests with arterial blood gas 
6. Radiation therapy consult 
7. Nutrition service consult 
8. Social work/psychiatry liaison consultation and 
family meeting 
8.2 Laboratory Evaluation 
1. CBC, platelet count, reticulocyte count, 
differential, type and screen/ direct Coomb's test, PT 
and PTT 
2. Complete serum chemistry panel to include 
electrolytes (including calcium, phosphate and 
. magnesium) , glucose, BUN, creatinine, total and direct 
bilirubin, alkaline phosphatase, SGOT/SGPT, LDH, beta-2 
microglobulin, albumin, total protein, serum protein 
electrophoresis and quantitative immumoglobulins 
3. 24 hour urine for creatinine clearance, urine 
protein electrophoreses' and quantitation. Routine 
urinalysis and urine culture. 
Recombinant DNA Research, Volume 16 
