4. HLA-typing (A and B antigens) and anti-HLA 
serologies 
5. HIV antibody testing (within 4 weeks prior to marrow 
harvest) 
6. Hepatitis A, B and C serologies 
7 . HSV, EBV and CMV serologies 
8. Bilateral bone marrow biopsy and aspiration to 
ensure adequate cellularity for harvest, to rule out 
extensive fibrosis, and to determine percentage plasma 
cells. Cells will also be used for molecular analysis 
of immunoglobulin gene rearrangements, in vitro growth 
analysis, cell surface antigen analysis and retroviral 
infectivity analysis. 
9 . 0 ON- STUDY EVALUATION 
9.1 Beginning of Pre-apheresis Chemotherapy until Marrow 
Engraftment Post-Transplantation 
1. Daily weights and input and output recording while 
hospitalized. 
2. Daily CBC, platelet count, differential, 
electrolytes, glucose, BUN, creatinine while 
hospitalized, then 2X/week until full engraftment 
(defined as transfusion-independence, ANC > 1000/ul off 
G-CSF, and platelets > 80,000/ul). 
3. Three times per week Ca++, P04++, Mg++, albumin, 
total protein, reticulocyte counts, SGOT, SGPT, 
bilirubin, LDH, alkaline phosphatase while 
hospitalized, then weekly until engraftment. 
4. -Weekly chest X-ray, urinalysis until hospital 
discharge. Daily urinalysis after cyclophosphamide X 1 
week . 
5. Q3D type and screen until' transfusion independent. 
6. §erum protein electrophoresis, quantitative 
immunoglobulins, urine protein electrophoresis, beta-2 
microglobulin day +30, then q month X 1 year. 
7. Bone marrow aspiration and 'biopsy at days +5, +10 
and +15 after 5FU, then weekly after transplantation 
until engraftment to assess engraftment and tumor cell 
percentage, as well as to obtain samples for PCR, 
Recombinant DNA Research, Volume 16 
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