Sch emq 
4 -5 Cycles Induction Chemotherapy 
Stable or PD 
PR or CR 
Other Therapies 
<1x1 0& buffv coat cells/Ko 
Bone Marrow harvest 
>1x10 8 buffv coat cells/Ko 
consider for transplant on 
another Medicine Branch 
study or consider 
other therapies 
30% used to 
separateCD34+ 
fraction 
70% 
cryopreserved 
(at least 
5*1 07/kg) 
CD34+ fraction transfected 
with the neo gene 
Sample peripheral blood and bone 
marrow at multiple time points during 
and after engraftment to test for 
presence of neo gene 
CR 
ICE chemotherapy 
administered 
Reinfuse transduced and 
non-transduced BM cells 
Begin daily G-CSF 
Restage Breast CA 1 
month post-ABMT 
Residual Disease 
Follow for presence 
of neo gene in BM and wbc and 
for breast CA recurrence 
Treatment with Vinblastine 
±G-CSF 
Relapse 
Biopsy relapsed tumor to 
test tumor for neo gene 
[90] 
Recombinant DNA Research, Volume 16 
