5.1 Bone Marrow Harvest 
Patients' bone marrow will be harvested after 4-5 cycles of 
induction chemotherapy and after patients' bone marrows have 
returned t o steady state aft er c hemot hera py- At least 2 weeks 
will elapse from blood count recovery from induction therapy 
and bone marrow harvest (see Section 6.01 . Bone marrow 
harvest is arranged by contacting Dr. Wyndham Wilson (496- 
6404 or page 104-2399-7) or George Bryant (496-9521 or 
page 104-2556-7) at least 1 week before the procedure. 
Patients must have an AGC>1 500/p.l and platelets >75,000/p.l. 
Marrow collection is performed in the Clinical Center 
operating rooms under general anesthesia. The patient must be 
admitted to the hospital by 2:00 P.M. on the day prior to 
marrow collection and evaluated by the anesthesiologist and 
BMT team. ABMT requires a minimum of 5 x 10Z buffv coat 
celis/kfl.. b Qdy we ight. In general. > 1x103. buffv coat cells/kg 
body weight are harvested. Every attempt will be made to 
harvest >3x108. buffv coat cells/Ko body weight before 
proces sing, If it is possible to obtain >1x1 0& buffy coat 
cells/Ka after Ficoll processing. 70% of the purified marrow 
will be immediately crvopreserved using standard techniques. 
The remaining 30% will be used for retroviral transfection. |f 
<1 x IPS, buffv coat cells/Ko body weight are harvested, the 
entire purified marrow will be frozen immediately. These 
patients will not be transplanted on this gene transfer pilot 
study, but may be eligible for ABMT with ICE chemotherapy and 
interleukin-1 or another ongoing Medicine Branch ABMT study. 
This option will be discussed with Dr. Wilson. 
5.1.1 The following preoperative tests must be obtained prior to 
harvest: CBC, platelets, PT, PTT, SMAC, urinalysis, Chest x- 
ray, ECG. Type and Cross 3 units irradiated PRBC for the CR. 
5.2 Bone Marrow Processing : 
Marrow will be filtered to remove bony particles and 
transported to the blood bank for further processing in sealed, 
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