sterile collection bags. Samples will be taken for counting, 
microbial culture, in vitro culture and surface antigen 
analysis. The total bone marrow will be processed with a 
Fenwal CS3000 automated separation unit to first purify a 
buffy coat preparation, then a monoclear cell fraction by 
density gradient centrifugation. Samples will again be taken 
for counting, in vitro culture, microbial culture and surface 
antigen analysis. 
6.0 Treatment Plan : 
6.1 Patient Registration: All patients must be registered on study 
by the clinical associate by contacting Orkand at 402-1732 
(8:30 A.M. - 5:00 P.M., Monday - Friday) with the appropriate 
- patient information. Patients must also be officially taken off 
study by contacting Orkand Personnel. 
6.2 All patients will have a double or triple lumen semi-permanent 
central venous catheter place in the operation room at the 
time of marrow harvest or on another occasion prior to 
initiation of high dose chemotherapy. 
6.3 Patients with metastatic breast cancer will be treated with 4- 
5 cycles of induction chemotherapy on an NCI Medicine Branch 
Treatment protocol which includes GM-CSF or G-CSF to 
simulate bone marrow recovery. Patients achieving at least a 
partial response with induction chemotherapy will be eligible 
for transplant. Patients who have not achieved a PR but who ar 
responding after 4-5 cycles may be treated with additional 
cycles in an attempt to achieve a PR. Such patients will be 
eligible for transplant if a PR is obtained. Patients who have 
achieved a PR after 4-5 cycles and who continue to respond 
mav be treated with additional cycles until a CR or best 
response is achieved. A maximum of 9 induction cycles will be 
administered. 
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