2. All patients will have a formal infectious disease 
consultation at the time of admission for 
transplant. 
6.6 Infection Prophylaxis/Oral Hygiene 
Routine antibiotic/oral hygiene orders for all patients 
include: 
Antibiotics: 
1. Bactrim DS 1 tab BID beginning day -6 to day +3. 
Patients will receive one double strength tablet 
bid on Saturday and Sunday until day +90. If 
unable to tolerate po, patients will receive 5 
mg/kg IV bid instead. 
2. Peridex solution, swish and spit q 4h, beginning 
day -4, d/c when ANC > 1000/mm 3 . 
3. Nystatin oral suspension 1 x 10 6 units P.O. q~4h 
begin day -10; D/C when ANC > 10 00 /mm 3 . 
4. Acyclovir 250 mg/m 2 I.V. ql2 h beginning day -5 
through day +30 for patients with a history of 
herpes simplex infections or positive herpes 
simplex antibody screen. 
Oral hygiene: 
1. Salt and soda solution , swish and spit q4hours, 
beginning day -10, D/C when ANC > 1000/mm 3 . 
2. Parenteral narcotics (including morphine sulfate 
continuous infusion or via PCA pump) if necessary 
for control of mucositis pain. 
7 . 0 Autologous Bone Marrow and Peripheral Blood Stem Cell 
Harvesting, Cell Separation and Cryopreservation of Cells 
7.1 Collection of Peripheral Blood Stem Cells: 
For leukopheresis of large quantities of peripheral 
blood, peripheral blood is removed via continuous flow 
centrifugation by means of a Fenwall CS3000 blood cell 
separator (Fenwal) , USA). A minimum of 3.0 x 10 8 
nucleated cells per kilogram body weight will be 
obtained. Collections will begin when the WBC is 
1000/mm 3 during the recovery phase from chemotherapy 
and G-CSF in patients with a marrow aspirate specimen 
which shows less than 10% blasts (see 11.5). 
Collections will continue until an adequate number of 
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Recombinant DNA Research, Volume 16 
