cytoreductive therapy when the WBC reaches 
1000/mm 3 ; 
9.3 Studies Prior to 5-FU and Marrow Harvest 
1. Prior to 5-FU: see on-study evaluation flow 
diagram, appendix H) A marrow 
aspirate/cytogenetics and biopsy will be obtained 
prior to initiating 5-FU therapy. 
2. A marrow aspirate and biopsy will be obtained 5 
days following the initial dose of 5-FU. 
3. Bone marrow harvest will be performed 10 days 
after initial dose of 5-FU. Routine pre-harvest 
admission labs: see Appendix H. A marrow 
aspirate/cytogenetics/biopsy will be obtained 
during the harvest while the patient is 
anesthetized. 
9.4 Studies Prior to Admission for Transplant 
Clinical Evaluation ■ 
1. History and physical examination with height and 
weight and body surface calculations. 
2. Chest and other x-rays as clinically indicated. 
3 . Marrow aspiration and biopsy within 2 weeks prior 
to admission for staging, cytogenetics, clonogenic 
assays, PCR, FACS. 
4. EKG 
5. Pulmonary function tests with arterial blood gases 
(spirometry, DLCO) . 
6. Cardiac Gated blood pool scan (MUGA) 
Laboratory Evaluation 
1. CBC, platelet count, reticulocyte count, 
differential, type and screen, direct Coomb's, 
PTT, Prothrombin time 
2. Ca, Mg, P04, glucose, BUN, total protein albumin, 
electrolytes, LFT's and creatinine. 
3. HIV antibody (results available prior to 
admission) 
Recombinant DNA Research, Volume 16 
[167] 
