Recombinant DNA Advisory Committee - 09/14-15/92 
regarding the ability to reconstitute following G-CSF administration. Dr. Schuening 
noted that his laboratory has conditioned 12 patients with G-CSF, harvested autologous 
peripheral blood cells, and transplanted the stem cells. All twelve of these patients 
recovered within the expected time frame. Dr. Leventhal asked if these patients 
received chemotherapy conditioning. Dr. Schuening stated that these patients did not 
receive chemotherapy, only G-CSF. 
A motion was made by Dr. Krogstad and seconded by Dr. Parkman to approve the 
identical twin protocol. Dr. Murray called for a vote. The motion passed by a vote of 
11 in favor, 2 opposed, and 5 abstentions. 3 
VIII. ADDITION TO APPENDIX D OF THE NIH GUIDELINES REGARDING THE 
INTRODUCTION OF A GENE CODING FOR TETRACYCLINE RESISTANCE INTO 
PORPHYROMONAS GINGIVALIS/ DR. PROGULSKE-FOX 
Review-Dr. Schaechter 
Dr. Murray called on Dr. Schaechter to present his primary review of the proposal 
submitted by Drs. Progulske-Fox and Keierleber of the University of Florida, Gainesville, 
Florida. Dr. Schaechter presented an overview of the proposal. The investigators are 
requesting to clone the gene encoding for tetracycline resistance into Porphyromonas 
gingivalis. The basis for this request comes from Section III-A-3 of the NIH Guidelines 
which states, Deliberate transfer of a drug resistance trait to microorganisms that are not 
known to acquire it (2) if such acquisition could compromise the use of the drug to control 
disease agent in human or veterinary medicine or agriculture. Since this request falls within 
this category, RAC, NIH, and IBC approval are required. This experiment should be 
approved because it carries a negligible risk. Although transfer of tetracycline markers 
to this organism (also known as Bacteroides gingivalis) has not been demonstrated, 
analogous transfers have been performed among similar Bacteroides from the human 
mouth. 
The use of tetracycline for the treatment of oral gingivitis is rooted in habit. It is 
doubtful that antibiotics in any way interfere with the course of this disease. The 
treatment of choice is surgical; and if antibiotics are used, there are other drugs from 
which to choose. Therefore, the transfer of tetracycline resistance to this organism 
mimics a natural phenomenon, and the acquisition of this trait is not likely to 
compromise the use of this drug to control human disease. 
Review— Dr. Krogstad 
3 Dr. D. Miller abstained. 
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