Table I 
Effects of treatment with Canine G-CSF (10 /ig/kg/day) on 
peripheral blood progenitor cells 
„ G-CSF ► 
Days Post 
G-CSF 
Pre 
1 
5 
8 
12 
14 
16 
18 
CFU-GM 
16 
131 
. 
69 
. 
42 
1398 
- 
X 10 3 
MNC 
87 
33 
161 
157 
205 
900 
• 
147 
BFU-E 
0 
16 
• 
10 
• 
0 
73 
_ 
X 10 3 
0 
0 
3 
23 
0 
10 
- 
0 
CFU-Mix 
0 
3 
• 
13 
. 
0 
46 
• 
X 10 3 
MNC 
0 
0 
3 
40 
10 
13 
• 
10 
(2) Collection of PBSC’s From Patients with Malignant Disease. 
Four patients who did not have marrow available for transplantation were 
transplanted with PBSC’s alone. Two patients received Cy and GM-CSF followed 
by collection of PBSC’s. They then were given G-CSF (16 mg/kg X 7 days) 
followed by a second set of PBSC collections on days 4-7. Two patients had 
PBSC’s collected following G-CSF alone. For engraftment the PBSC’s from both 
sets of collection were utilized in patients 1 and 2. The following table shows the 
results of PBSC collections following Cy and GM-CSF and/or G-CSF alone. 
Table H 
PBSC Collections Following Cy and GM-CSF 
and/or G-CSF (16 /ig/kg X 7 days) 
Total TNC MNC CD34+ CFU-GM 
Collections X 10*/kg X 10*/kg X 10‘/kg X ltf/kg 
l.Cy + GM-CSF = 4 
4.6 
4.4 
15.6 
84.5 
G-CSF = 5 
24.4 
14.9 
14.1 
76.7 
2.Cy + GM-CSF = 4 
3.7 
3.4 
12.8 
41.6 
G-CSF = 5 
34.1 
11.6 
16.9 
33.2 
3. G-CSF = 4 
12.7 
7.7 
4.3 
4.5 
4. G-CSF = 5 
20.4 
19.1 
14.6 
103.8 
[362] 
Recombinant DNA Research, Volume 16 
