THE FRED HUTCHINSON CANCER RESEARCH CENTER 
AND THE UNIVERSITY OF WASHINGTON SCHOOL OF MEDICINE 
DEPARTMENT OF MEDICINE, DIVISION OF ONCOLOGY 
1. Consent to Participate in A Trial of G-CSF Stimulated Peripheral Blood Stem Cells for 
Engraftment in Identical Twins - Recipient 
INVESTIGATORS: CD. Buckner, MD., Professor of Medicine, UW, Member, FHCRC (667-4453) W. 
Bensinger, MD. Associate Professor of Medicine, UW, Associate Member, FHCRC, (667-4933); F. 
Schuening, M.D., Assistant Member, FHCRC (667-4410); J. Singer, MD.; R. Storb, MD., Professor of 
Medicine, UW, Member, FHCRC, (667-4407); S. Rowley, M.D., Associate Member, FHCRC, (667- 
5914); P. Martin, M.D., Professor of Medicine, UW, Member, FHCRC, (667-4798); L. Fisher, PhD., 
Professor of Biostatistics, UW, Member, FHCRC, (667-4567). 
Emergency (24 hour) Phone: 667-5001 
Attending physician: Phone: 
BACKGROUND 
This marrow transplant procedure is being undertaken in an attempt to cure your cancer.. During this 
treatment you will undergo intensive chemotherapy and radiation treatments. These treatments in 
addition to killing your cancer will result in irreversible damage to your bone marrow. Without a 
replacement source of stem cells, you would die. Stem cells reside in your bone marrow and produce 
blood cells. The usual source of stem cells is marrow that is harvested from your identical twin. More 
recently, doctors have used growth factors (substances that stimulate stem cells in the bone marrow) to 
cause stem cells to circulate in the peripheral blood. This allows the collection of the cells by 
leukapheresis equipment (special machines that collect cells from the blood). It has been found that by 
using these growth factor stimulated peripheral blood stem cells, a bone marrow harvest can be avoided 
and the recovery of blood counts after infusion of these cells is more rapid than with bone marrow 
alone. 
PURPOSE 
The purpose of this study will be to utilize these growth factor stimulated peripheral blood stem cells in 
order to determine whether these cells are capable of engraftment without the use of bone marrow. 
There is no guarantee or promise that the procedures will be successful. Your bone marrow donor will 
be given five days of injection with a growth factor called G-CSF. Leukapheresis procedures will be 
performed for three days beginning on day zero, the usual day of the marrow harvest. The fresh 
collections of stem cells will then be transfused through your Hickman catheter for three consecutive 
days. 
PROCEDURES 
During the first 2-3 weeks postgrafting, approximately 10 to 100 ml of blood (2 teaspoons to 20 
teaspoons ) will be drawn daily or less frequently depending on your condition. The maximum amount 
of blood drawn per day will not exceed 5 ml/kg patient weight (1 tsp/kg). Blood will be drawn less 
frequently as you progressively improve. These blood samples will be used for various tests to monitor 
Recombinant DNA Research, Volume 16 
[373] 
