The next procedure will require the insertion of a catheter into a large vein in your chest This 
procedure takes about 15 minutes and is done under a local anesthetic and will be done in the same 
room where the collection of peripheral blood cells will be performed. The peripheral blood stem cell 
collections, called leukapheresis, will be performed daily for three days beginning on the 4th, 5th, and 
6th day after beginning the G-CSF injections. Procedures will require three to four hours each for 
three consecutive days. 
During the first 2-3 weeks postgrafting, approximately 10 to 100 ml of blood (2 teaspoons to 20 
teaspoons) will be drawn daily or less frequently depending on your condition. The maximum amount 
of blood drawn per day will not exceed 5 ml/kg patient weight (1 tsp/kg). Blood will be drawn less 
frequently as you progressively improve. These blood samples will be used for various tests to monitor 
the your clinical condition. Special studies involving blood drawing for specific research studies are 
described in the appropriate consent forms. 
RISKS, STRESS, OR DISCOMFORT 
There is a minor amount of discomfort from the daily injections with G-CSF. Occasional patients 
complain about bone aches on the 3rd or 4th day of the injections. The placement of the catheter in 
the chest will result in some minor discomfort or soreness for a few days. There is the possibility of 
bleeding from this catheter. The leukapheresis procedure may result in some numbness or tingling in 
the jaw or fingers because of one of the anticoagulants used. This can be easily treated with calcium 
infusions. 
It is possible that if the peripheral blood stem cells do not result in a bone marrow graft, it will still be 
necessary to harvest your bone marrow. This would occur 20-30 days following completion of your 
peripheral blood collections. 
BENEFITS 
The benefits are that if successful, you would avoid the necessity for a bone marrow harvest. The other 
benefit is that your twin may achieve faster recovery of blood counts and earlier discharge from the 
hospital. This would also lessen the chance of infection or bleeding after transplant. 
ALTERNATIVES 
The alternatives are not to participate in this study in which case your marrow would be harvested in 
the usual manner. 
OTHER INFORMATION 
Your decision to participate in this study is voluntary. You can decide whether or not to participate in 
this study. You may decide not to participate in this study at any time, for any reason, without notice. 
There is no financial compensation for participation in this program. 
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Recombinant DNA Research, Volume 16 
