following the end of the mesna infusion. VP16, 
125 mg/m , bid for three days will be given days 1 
to 3. 
The hydration given along with the 
cyclophosphamide is 4 liters every 24 hours as 
tolerated. We will supplement this as necessary 
to maintain intravascular fluid volume and urine 
output . 
Total body irradiation (TBI) is 1020 centigrays. 
The patients are placed in the supine position 
and the TBI is directed from the right side with 
a calculated mid plane dose of 200 rads/fraction, 
each fraction even bid starting day 6 to 8 . 
b. Autologous peripheral blood and bone marrow will 
be reinfused on day 9 after the last dose of TBI 
after premedication with 25 mg of benadryl and 
100 mg of solucortef 30 minutes before reinfusion 
to prevent anaphylactic reactions. 
c. Treatment of patients in the Protected 
Environment on the 12th floor of the Lutheran 
Pavilion is strongly urged. 
Patients will remain there until the attainment 
of 500 granulocytes/mm 3 . 
d. Patients will receive bacterial (norf laxacin) and 
fursfol/yeast (fluconazole) antibiotic 
prophylaxis or other antibiotics specified by the 
Infectious Disease group while hospitalized to 
protect patients against overwhelming infection. 
e. All blood products will be irradiated from the 
start of treatment and for three months following 
transplantation . 
f. Back-up marrow will be given if the total ANC is 
still less than 500 at day 50. 
Schema for Treatment Plan: 
I. Conventional dose combination on chemotherapy. 
II. Collection of peripheral blood cells as back-up 
for transplant. 
III. Collection of autologous cells from marrow, and 
in vitro purging. 
IV. Exposure of marrow to CD34 selection and the CD19 
selection marrow cells to the LNL-6 and GIN 
viruses, respectively. PCR for Neo for the 
immunoglobulin gene rearrangement will be done 
before and after marking. 
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Recombinant DNA Research, Volume 16 
