400 r 
Figure 1 : Functional transduction efficiency of LNSX-AS-K -ras in H460a cells. Growth 
curves are shown for 10 3 cells/well seeded in 12 well plates. H460a cells were 
infected by incubation 0.5 m of viral supernate stock from either LNSX or LNSX-AS-K- 
ras (6X1 0 6 CFU/ml) daily for 4 consecutive days in the presence of 8/vg/ml of 
polybrene. The parental H460a cells served as a control. Cells were not selected 
with G418. Cells were counted daily. The mean ± SE is shown for 3 replicates. 
Figure 2: H460a cells were infected with LNSX-AS-K -ras by incubating 10 4 cells with 
0.5 ml of viral stock (6X1 0 6 CFU/ml) produced by the packaging cell line 
GP + envAm12 in the presence of 8/yg/ml of polybrene. Infection was done daily foM 
to 7 days. Two days later cells were plated in equal numbers into selective media 
containing 200/yg/ml G418. Control H460a cells were plated at equal cell numbers to 
determine baseline colony forming efficiency. The infection efficiency was measured 
by determining the percent of the unselected colony number formed by the G418 
selected colonies. 
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Recombinant DNA Research, Volume 16 
