Figure 2. 3 -A. Genomic organization of AvlCFl and AdCFTR compared to Ad5 . 
Shown (top to bottom) are a map of the Ad5 genome indicating the regions 
deleted in AvlCFl and AdCFTR and overall maps of AvlCFl and AdCFTR. The 
regions of Ad5 not present in AvlCFl and AdCFTR include the entirety of Ela 
and 69. 5Z of the left end of Elb and 66Z of E3 . The deletion of the El 
region is replaced with the Ad5 major late promoter (MLP; from Ad5 in 
AvlCFl and from Ad2 in AdCFTR), the Ad tripartite leader sequences 1, 2, 
and 3 (from Ad5 in AvlCFl and from Ad2 in AdCFTR) and the human CFTR cDNA. 
AdCFTR includes an SVAO-derived polyA signal after the CFTR cDNA; AvlCFl 
uses the Elb polyA signal (indicated). ITR — inverted terminal repeat. Not 
shown - the minor 2 bp deletion of the VA-I promoter (in the region of 29 
m.u.) in both AvlCFl and AdCFTR. 
Map units 
0 10 20 30 40 50 60 70 
n 1 1 i 1 1 1 r 
80 90 100 
n 1 n 
101 
1.25 9.2 78.5 84.7 
I 
SV40 
polyA 
[660] 
Recombinant DNA Research, Volume 16 
