Table 2.3-B 
Structural Elements Contained in the Genome of AdCFTR and AvlCFl 
Element 
AdCFTR 
AvlCFl 
Differences 
ITR origin of 
Replication 
Ad5 
Ad5 
None 
\J> + El promoter/ 
enhancer 
Ad5 
Ad5 
AvlCFl includes 11 bp more of the 
El promoter 1 
Major late 
promoter 
Ad2 
Ad 5 
2 bp 2 
Tripartite 
leader 
Ad2 
Ad5 
Minor sequence differences in 
3rd tripartite leader 2 
CFTR gene 5' 
untranslated region 
11 bp 
61 bp 
AdCFTR contains less 5' untrans- 
lated region 3 
CFTR cDNA coding 
sequence 
Normal 
Normal 
Minor differences 3 
CFTR gene 3 ' 
untranslated region 
12 bp 
150 bp 
AdCFTR contains less 3' untrans- 
lated region 3 
SV40 early region 
polyA signal 
Yes 
No 
i 
AdCFTR but not AvlCFl contains 
SV40 polyA signal 4 
Ad5 right end 5 
From 9 . 24 
m.u. to 
right end 6 
From 9 . 24 
m.u. to 
right end 6 
None 

x Ad5 Ela promoter sequences 455-466 are included in AvlCFl, not in AdCFTR 
2 GenBank accession //M73260 
3 For AdCFTR see Appendix 1 and Yoshimura et al. , 1991b; for AvlCFl see Appendix 3 
a SV 40 early region 2666-2538 bp (GenBank accession # V01380) 
5 Most of E3 region deleted and a 2 bp deletion of the VA-I promoter, 
6 Appendix 3 
see 
section 
[ 662 ] 
Recombinant DNA Research, Volume 16 
