pS2 CFTR construction plasmid 
Major late 
promoter 
Elb polyadenylation signal 
Tripartite 
• Origin of leader 
replicaiton 
• Encapsidation 
signal (i|i) 
• El a promoter/enhancer 
Adenoviral DNA 
for homologous 
recombination 
Figure 2.4-B. Schematic of the adenoviral construction plasmid pS2CFTR used 
in the final construction of AvlCFl. pS2CFTR was constructed by inserting 
the CFTR cDNA into the base plasmid pS2 (see Figure 2.4-A). Shown are left 
end genomic elements of Ad5 including the left inverted terminal repeat 
(ITR) and origin of replication. This is followed by the Ela enchancer and 
encapsidation signal (V>) , the major late promoter, tripartite leader, and a 
segment of adenovirus type 5 [from position 9.24 m.u. (3328 bp) to 17.34 
m.u. (6241 bp)] used for homologous recombination. 
Recombinant DNA Research, Volume 16 
[665] 
