Figure 3.1.1-A. Evaluation of Ad- CFTR- directed human CFTR transcripts in 
human bronchial cells by in situ hybridization. Human bronchial brushing 
cells (freshly recovered during bronchoscopy by cytologic brush from normal 
individuals) were infected with Ad- CFTR for 48 hr (Appendix 1; Rosenfeld et 
al . , 1992). Human CFTR mRNA transcripts were evaluated in cytocentrifuge 
preparations by in situ hybridization with 35 S- labeled antisense (A,B,C,) 
and sense (D) CFTR RNA probes. A. Uninfected cells (control). B-D. Ad-CFTR 
infected cells . ( 
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