Figure 3.1.1-B. Evaluation of Ad- CFTR- directed human CFTR transcripts in 
human bronchial cells by in situ hybridization. Human bronchial brushing 
cells (freshly recovered during bronchoscopy by cytologic brush from indi- 
viduals with CF) were infected with Ad- CFTR for 24-48 hr (Appendix 1; 
Rosenfeld et al . , 1992). Human CFTR mRNA transcripts were evaluated in 
cytocentrifuge preparations by in situ hybridization with 35 S- labeled sense 
(A) and antisense (B,C,D) CFTR cRNA probes. Shown are Ad- CFTR infected 
cells from individuals with CF using direct light microscopy (left) and 
dark field illumination (right) . Uninfected cells had no transcripts evi- 
dent when evaluated with the antisense probe (not shown) . 
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Recombinant DNA Research, Volume 16 
