A B C 
Figure 3. 1.2 -A. Immunohistochemical detection of CFTR protein in non-human 
primate (rhesus) respiratory epithelial cells after infection with Ad-CFTR. 
Rhesus bronchial brushing cells (freshly recovered during bronchoscopy by 
cytologic brush) were infected in vitro with Ad-CFTR and collected 24 hr 
later (Appendix 1) . Human CFTR protein was evaluated in cytocentrifuge 
preparations using the alkaline phosphatase anti-alkaline phosphatase 
method with minor modifications and anti-human CFTR antibody as previously 
described (Appendix 1) . Shown are control uninfected cells (A) , cells 
infected with Ad-CFTR (B) and as a control, cells infected with Ad-CFTR 
evaluated with an irrelevant primary antibody (C) . Immunoreactivity to 
human CFTR is indicated by a red color; cell nuclei appear blue as a result 
of hematoxylin counters taining. 
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