Figure 3.1.2-B. Inununohistochemical detection of CFTR protein in human 
respiratory epithelial cells after infection with Ad-CFTR. Human bronchial 
brushing cells (freshly recovered during bronchoscopy by cytologic brush 
from individuals with CF) were infected in vitro with Ad-CFTR and evaluated 
at various time points (Appendix 1; Rosenfeld et al . , 1992). Human CFTR 
protein was evaluated in cytocentrifuge preparations using the alkaline 
phosphatase anti -alkaline phosphatase method with minor modifications and 
anti -human CFTR antibody as previously described (Appendix 1) . Shown are 
control uninfected cells cultured for 30 hours (A) and cells infected with 
Ad-CFTR for 30 hours (B) . Panel C shows four individual ciliated cells 
infected with Ad-CFTR for 48 hours; in the lower right corner of panel C, 
as a control, the primary antibody was eliminated. Immunoreactivity to 
human CFTR is indicated by a red color; cell nuclei appear blue as a result 
of hematoxylin counters taining . 
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