Ad-alAT Ad-CFTR 
Figure 3.1.2-D. Detection of de novo synthesized CFTR protein in respirato- 
ry epithelial cells after infection with Ad-CFTR. Human bronchial brushing 
cells (freshly recovered during bronchoscopy by cytologic brush from normal 
individuals) were infected in vitro with Ad-CFTR or the control virus Ad- 
alAT (Appendix 1), simultaneously labelled with [ 35 S ] methionine and evalu- 
ated 48 hr later. Cell lysate was prepared and evaluated for [ 35 S] methio- 
nine labelled CFTR by immunoprecipitation, sodium dodecyl sulfate-poly- 
acrylamide gel electrophoresis and autoradiography as previously described. 
Shown are cells infected with the control adenovirus vector (see Rosenfeld 
et al. , 1991) Ad-alAT (lane 1) and Ad-CFTR (lane 2). The size range for 
CFTR is indicated. 
Recombinant DNA Research, Volume 16 
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