Figure 3.1.3-A. Jn vitro evaluation of the function of human CFTR protein 
directed by AvlCFl . The functional ability of AvlCFl to modulate forskolin- 
stimulated Cl - permeability was evaluated in CFPAC-1 cells [pancreatic 
adenocarcinoma cells from an individual homozygous for the common aF508 
mutation (Rommen et al. , 1989; Riordan et al . , 1989; Kerem et al., 1989; 
Collins, 1992)]. CFPAC-1 cells were trypsinized, counted, seeded (3 cm 
plates [5x10 s cells/plate]) and infected in suspension at 200 pfu/cell with 
AvlCFl or AvlNull (a control vector identical to AvlCFl except that the 
CFTR cDNA is deleted) . Cl" efflux was evaluated 48 hr after infection at 
rest (basal) and after stimulation (forskolin) as previously described 
(Appendix 1; Trapnell et al., 1991b) in uninfected CFPAC-1 cells (A), cells 
infected with AvlNulll (B) and cells infected with AvlCFl (C) . 
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