Figure 3.1.3-B. In vitro evaluation of the function of human CFTR protein 
directed by AdCFTR. The functional ability of AdCFTR to modulate forskolin- 
stimulated Cl - permeability was evaluated in IB3-1 cells [epithelial cell 
line derived from an individual with cystic fibrosis (Zeitlin et al. 1991)] 
IB3-1 cells were trypsinized, counted, seeded (3 cm plates [ 5xl0 5 
cells/plate]) and infected in suspension at 200 pfu/cell with AdCFTR or a 
control vector (AvlNull, an E1~E3“ repliction deficient adenovirus identi- 
cal to AvlCFl , but with no CFTR cDNA. Cl - efflux was evaluated 48 hr after 
infection at rest (basal) and after stimulation (forskolin) as previously 
described (Appendix 1; Trapnell et al., 1991b) in (A) cells infected with 
AvlNull and (B) IB3-1 cells infected with AdCFTR. 
Ad-CFTR 
£ 
Epithelial cell line 
from CF airway (IB3-1) 
I 
48 hr 
Evaluate 
• CFTR function (Cl permeability 
in response to increased cAMP) 
A. Control virus B. Ad-CFTR 
Time (min) 
[684] 
Recombinant DNA Research, Volume 16 
