Media I I — + CF ELF 
Figure 3.1.4-A. Effect of epithelial lining fluid of individuals with 
cystic fibrosis on the in vitro expression of a recombinant, replication 
deficient adenovirus vector. AdRSV.^gal (10 7 pfu) was incubated (30 min, 
37°) with media or epithelial lining fluid (ELF) from an individual with 
cystic fibrosis. The various fluids were then added to HeLa cells (10 6 
cells, 10 pfu/cell) and the incubation continued for 24 hrs at 37°. 
galactosidase activity was evaluated in cell lysates by spectrophotometry 
(420 nm) ; see Appendix 2 for details regarding the vector and assay meth- 
ods) . 
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