A 
B 
Figure 3.2.2-A. Histochemical detection of E , Coli S-galactosidase in rhesus 
monkey respiratory epithelial cells after in vivo infection with 
AdRSV.Rgal. Rhesus monkey tracheal epithelial cells were infected in vivo 
with 2xl0 9 pfu/kg of an adenovirus vector derived from type 5 adenovirus 
and containing the Eh. coli lacZ gene which codes for the S-galactosidase 
protein (see Appendix 2). After 3 days, epithelial cells were recovered 
from the trachea by bronchoscopic visualization and removal using a cytolo- 
gy sampling brush. S-galactosidase was evaluated in cytocentrifuge prepara- 
tions using X-gal as a substrate. Samples were stained with hematoxylin and 
eosin. Shown are: (A) epithelial cells obtain from a control animal admin- 
istered vehicle; and (B) epithelial cells obtained from an animal adminis- 
tered AdRSV.Sgal. The presence of S- galactosidase is indicated by a dark 
blue nuclear staining. 
Recombinant DNA Research, Volume 16 
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