Table 4.9-C. Assessment of nasal epithelium, bronchial epithelium, and 
inflammatory cells on the respiratory epithelial surface of individuals 
with cystic fibrosis for the presence of respiratory viruses. Individuals 
with stable cystic fibrosis (not during an exacerbation of respiratory 
symptoms), normals, individuals with al-antitrypsin deficiency, and indi- 
viduals who smoke cigarettes (but are otherwise normal) underwent fiber- 
optic bronchoscopy with sampling of respiratory epithelium by brushing 
nasal mucosa, brushing large airways (bronchial epithelium), or broncho- 
alveolar lavage (to recover inflammatory cells on the respiratory epitheli- 
al surface) by standard techniques. Samples were evaluated for virus by 
culturing on tissue culture cell lines in the presence of antibiotic and 
antifungal suppression. Specific viruses were identified by growth on the 
appropriate cell lines and confirmed by specific immunof luorescent staining 
for virus specific capsid proteins with FITC- conjugate anti-viral capsid 
monoclonal antibodies. Viruses evaluations included: cytomegalovirus (CMV) , 
varicella-zoster virus (VZV) , herpes simplex viruses I and II (HSV) , influ- 
enza viruses A and B (INF A,B), parainfluenza viruses 1, 2 and 3 (PARA 
1,2,3), respiratory syncytial virus (RSV) , and adenovirus (ADENO, including 
all human non-enteric types). All samples were evaluated in duplicate. Note 
that "INF A,B, + PARA 1,2,3" are detected by one culture system, while 
"RSV, ADENO + PARA 1,2,3" are detected by another culture system. Of all 
samples evaluated, virus was recovered from only 2 individuals with cystic 
fibrosis, with herpes simplex virus recovered from bronchial epithelium and 
lavage of one individual and lavage alone from a second individual. All 
other respiratory samples were culture negative for all viruses. 
Recombinant DNA Research, Volume 16 
[725] 
