M.J. Welsh and A.E. Smith, RAC Application 
a. Study One. 
Procedures 
This study was a time course assessment of the pulmonary and systemic acute 
inflammatory response to a single intratracheal administration of Ad2/BGal-l. In this 
study, a total of 24 animals were distributed among three treatment groups: 8 vehicle 
control, 8 low dose virus (IxIOH particles; 3x10^ PFU), and 8 high dose virus (1.7x10^ 
particles; 5x10^ PFU). Within each treatment group, 2 animals were analyzed at each of 
four time points after viral vector instillation; the times were 6 hrs, 24 hrs, 48 hrs, and 7 
Recombinant DNA Research, Volume 16 
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