60116 
NOTICES 
fact, experiments in this category may 
be performed with any E. coli K-12 
vector (e.g., conjugative plasmids). 
However, for prokaryotes that are 
classified [1] as Class 2, the contain- 
ment levels are P2 + EK1. 
III-A-l-b-t2). Prokaryotes That Do 
Not Exchange Genetic Information 
with E. Colt P2 physical containment 
+ an EK1 host-vector, or PI + EK2, 
except for DNA from Class 2 agents, 
[1] which require P3 + EK2. 
III-A-2. Plasmids, Bacteriophages, 
and Other Viruses. Recombinants 
formed between a vector and some 
other plasmid or virus DNA have in 
common the potential for acting as 
double vectors because of the replica- 
tion functions in these DNAs. The 
containment conditions given below 
apply only to propagation of the DNA 
recombinants in E. coli K-12 hosts. 
They do not apply to other hosts in 
which the recombinants may be able 
to replicate as a result of functions 
provided by the DNA inserted into the 
EK vectors. These are considered 
under other host-vector systems. 
III-A-2-a. Viruses of Eukaryotes 
(summary given in Table III). 
III-A-2-a-(l). DNA Viruses. 
III-A-2-a-(l)-(a). Nontransforming 
viruses. 
III-A-2-a-< 1 )-( a)-( 1 ). Adeno-Associ- 
ated Viruses, Minute Virus of Mice, 
Mouse Adenovirus ( Strain FL), and 
Plant Viruses. PI physical contain- 
ment -i- and EK1 host-vector shall be 
used for DNA recombinants produced 
with (i) the whole viral genome, (ii) 
subgenomic DNA segments, or (iii) pu- 
rified cDNA copies of viral mRNA. 
[37] 
III-A-2-a-( 1 )-(a)-( 2). Hepatitis B. 
III-A-2-a-(l)-(a)-(2)-(a). PI physical 
containment + an EK1 host-vector 
shall be used for purified subgenomic 
DNA segments. [38] 
III-A-2-a-(l)-(a)-(2)-(6). P2 physical 
containment + an EK2 host-vector, or 
P3 + EK1, shall be used for DNA for 
recombinants produced 'with the 
whole viral genome or with subgeno- 
mic segments that have not been puri- 
fied to the extent required in footnote 
38. 
III-A-2-a-(l)-(a)-(2)-(c). P2 physical 
containment + an EK1 host and a 
vector certified for use in an EK2 
system, or P3 + EK1, shall be used for 
DNA recombinants derived from puri- 
fied cDNA copies of viral mRNA. [37] 
III-A-2-a-(l)-(a)-(3). Other Non- 
transforming Members of Presently 
Classified Viral Families. [36] 
III-A-2-a-(l)-(a)-(3)-(a). PI physical 
containment + an EK1 host-vector 
shall be used for (i) DNA recombin- 
ants produced with purified subgeno- 
mic DNA[38] segments or (ii) purified 
cDNA copies of viral mRNA. [37] 
III-A-2-a-(l)-(a)-(3)-(b). PI physical 
containment + an EK1 host and a 
vector certified for use in an EK2 
system shall be used for DNA recom- 
binants produced with the whole viral 
genome or with subgenomic segments 
that have not been purified to the 
extent required in footnote 38. 
III-A-2-a-(l)-(b). Transforming Vir- 
uses. [37A] 
III-A-2-a-(l)-(b)-(f). Herpes Sai- 
miri. Herpes A teles, and Epstein Barr 
Virus. [39] 
III-A-2-a-( l)-(b )-(!)-( a). PI physical 
containment + an EK1 host-vector 
shall be used for DNA recombinants 
produced with purified nontransform- 
ing subgenomic DNA segments. [38] 
III-A-2-a-(l)-(b )-(!)-( b). P2 physical 
containment + an EK1 host and a 
vector certified for use in an EK2 
system, or P3 + EK1, shall be used for 
(i) DNA recombinants produced with 
purified subgenomic DNA segments 
containing an entire transforming 
gene [38] or (ii) purified cDNA copies 
of viral mRNA. [37] 
III-A-2-a-( 1 )-( b )-( I )-( c ). P3 physical 
containment + an EK1 host-vector, or 
P2 + EK2, shall be used for DNA re- 
combinants produced with the whole 
viral genome or with subgenomic seg- 
ments that have not been purified to 
the extent required in footnote 38. 
III-A-2-a-(l)-(b)-(2). Other Trans- 
forming Members of Presently Classi- 
fied Viral Families. [36] 
III-A-2-a-(lMb)-(2)-(a). PI physical 
containment + an EK1 host- vector 
shall be used for DNA recombinants 
produced with purified nontransform- 
ing subgenomic DNA segments. [38] 
Hl-a-2-a-(l)-(b)-(2)-(6). P2 physical 
containment + an EK1 host and a 
vector certified for use in an EK2 
system, or P3 + EK1, shall be used for 
(i) DNA recombinants produced with 
the whole viral genome, (ii) subgeno- 
mic DNA segments containing an 
entire transforming gene, (iii) purified 
cDNA copies of viral mRNA, [37] or 
(iv) subgenomic segments that have 
not been purified to the extent re- 
quired in footnote 38. 
III-A-2-a-(2). DNA Transcripts of 
RNA Viruses. 
III-A-2-a-(2)-(a). Retroviruses. 
III-A-2-a-( 2 )-(a)-( 1 ). Gibbon Ape, 
Woolly Monkey, Feline Leukemia and 
Feline Sarcoma Viruses. [39] 
III-A-2-a-(2)-(a)-(f )-(a). PI physical 
containment + an EK1 host- vector 
shall be used for DNA recombinants 
produced with purified nontransform- 
ing subgenomic DNA segments. [38] 
III-A-2-a-(2)-(a)-(I )-(&). P2 physical 
containment + an EK1 host and a 
vector certified for use in an EK2 
system, or P3 + EK1, shall be used for 
DNA recombinants produced with pu- 
rified subgenomic DNA segments [38] 
containing an entire transforming 
gene. 
III-A-2-a-(2)-(a)-(f )-(c). P2 physical 
containment + an EK2 host-vector, or 
P3 + EKI, shall be used for DNA re- 
combinants produced with (i) the 
whole viral genome, (ii) purified cDNA 
copies of viral mRNA, [37] or (iii) sub- 
genomic segments that have not been 
purified to the extent required in foot- 
note 38. 
III-A-2-a-(2)-(a)-(2). Other Members 
of the Family Retroviridiae. [36] 
III-A-2-a-(2)-(a)-(2)-(a). PI physical 
containment +- an EKI host-vector 
shall be used for DNA recombinants 
produced with purified nontransform- 
ing subgenomic DNA segments. [38] 
III-A-2-a-( 2 )-( a )-( 2)-( 6 ). P2 physical 
containment + an EKI host and a 
vector certified for use in an EK2 
system, or P3 + EKI, shall be used for 
DNA recombinants produced with (i) 
subgenomic DNA segments containing I 
an entire transforming gene, (ii) the 
whole viral genome, or (iii) purified 
cDNA copies of viral mRNA, [37] or 
(iv) subgenomic segments that have 
not been purified to the extent re- 
quired in footnote 38. 
III-A-2-a-(2)-(b). Negative Strand 
RNA Viruses. PI physical containment 
4- an EKI host-vector shall be used for| 
DNA recombinants produced with (i)| 
cDNA copies of the whole genome, (ii) 
subgenomic cDNA segments, or (iii) 
purified cDNA copies of viral mRNA. 
[37] 
III-A-2-a-(2)-(c). Plus-Strand RNA\ 
Viruses. 
III-A-2-a-(2)-(c)-(f ). Types 1 and 2 
Sabin Poliovirus Vaccine Strains and 
Strain 17D ( Theiler ) of Yellow Fever 
Virus. PI physical containment + and 
EKI host-vector shall be used for DNA 
recombinants produced with (i) cDNA 
copies of the whole viral genome, (ii) 
subgenomic cDNA segments, or (iii}! 
purified cDNA copies of viral mRNA 
[37] 
III-A-2-a-(2)-(c)-(2). Other Plus 1 II 
Strand RNA Viruses Belonging to Pres 
ently Classified Viral Families. [36] 
FEDERAL REGISTER, VOL. 43, NO. 247— FRIDAY, DECEMBER 22, 1978 
