3 
levels for these classes of experiments will require review by NIH, 
seme times after recommendation by the Recombinant DNA Advisory Committee 
(RAC). Hence, investigators are urged to provide to ORDA, in writing, 
full information on the proposed experiments prior to submission of a 
formal Memorandum of Understanding and Agreement (MUA)], 
I-B-l-b. Projects requiring the P4 level of physical containment, 
I-B-l-c. Reductions of more than one step in containment levels 
for characterized clones (see Section III-A-3 of the Guidelines), 
I-B-l-d. Reductions of containment levels for projects involving 
primate DNA (see Section III-A-3 of the Guidelines), 
I-B-l-e. Reductions of containment to levels below PI + EK1 for 
purified ENA and characterized clones (see Section III-A-3 of the 
Guidelines) , 
I-B-l-f. Initiation of the first project conducted in a facility 
at the P3 level of physical containment, or 
I-B-l-g. Initiation of the first recombinant ENA project subject 
to the Guidelines at an Institution. 
I-B-2. Requests for Activities Requiring Prior Approval . 
I-B-2-a. Activities Requiring Prior NIH Approval . MUAs incorporat- 
ing activities that require prior NIH approval must be submitted to NIH 
[ 66 ] 
