1 
2 
3 
4 
5 
6 
7 
8 
9 
10 
11 
12 
13 
14 
15 
16 
17 
18 
19 
20 
21 
22 
23 
24 
25 
46 
requirements . The possibility of using recombinant D1JA 
procedures for the development or improvement of entomo- 
pathogenic strains of bacteria for insect management has 
been considered by myself, and I have begun some of the 
basic research necessary to facilitate the use of bacteria 
towards this end. Envisioned are possible applications 
of _in vitro gene-splicing and recombinant-DNA-cloning to, 
one, expand the insect-pest host spectrum of existing 
insect pathogens; two, develop more potent strains of 
pest insect pathogens; three, improve the physiological 
tolerance and epidemiological properties of these 
bacteria; and four, facilitate the _in vitro commercial 
production of the more fastidious insect pathogens by 
expanding the range of substrates upon which they can 
grow. Two of the present organisms with which I am 
working, 3acillus popill iae , the Japanese beetle pathogen, 
and Bacillus thuringiensis , a pathogen of pest caterpillar 
larvae, both enjoy EPA registration and exemption from 
tolerance in the environment. 
Back in October of 1977 , a copy of my proposed 
recombinant DMA program was forwarded to the NIH Office 
of Recombinant DNA Activities for comment and clarification 
of certain aspects of the 1976 UIH guidelines dealing with 
shotgun experiments in prokaryotes. Apparently the UIH 
Recombinant DNA Advisory Committee was unable to come to 
[138] 
